Cell type-specific biotin labeling in vivo resolves regional neuronal and astrocyte proteomic differences in mouse brain

Proteomic profiling of brain cell types using isolation-based strategies pose limitations in resolving cellular phenotypes representative of their native state. We describe a mouse line for cell type-specific expression of biotin ligase TurboID, for in vivo biotinylation of proteins. Using adenovira...

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Detalles Bibliográficos
Autores principales: Rayaprolu, Sruti, Bitarafan, Sara, Santiago, Juliet V., Betarbet, Ranjita, Sunna, Sydney, Cheng, Lihong, Xiao, Hailian, Nelson, Ruth S., Kumar, Prateek, Bagchi, Pritha, Duong, Duc M., Goettemoeller, Annie M., Oláh, Viktor János, Rowan, Matt, Levey, Allan I., Wood, Levi B., Seyfried, Nicholas T., Rangaraju, Srikant
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9132937/
https://www.ncbi.nlm.nih.gov/pubmed/35614064
http://dx.doi.org/10.1038/s41467-022-30623-x

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9132937/
https://www.ncbi.nlm.nih.gov/pubmed/35614064
http://dx.doi.org/10.1038/s41467-022-30623-x

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