Endoglycosidase assay using enzymatically synthesized fluorophore-labeled glycans as substrates to uncover enzyme substrate specificities

Glycan synthesis and degradation are not template but enzyme only driven processes. Substrate specificities of glyco-enzymes determine the structures of specific natural glycans. Using endoglycosidases as examples, we describe methods to study these enzymes. Endoglycosidase S/S2 specifically deglyco...

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Detalles Bibliográficos
Autores principales: Wu, Zhengliang L., Ertelt, James M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9132957/
https://www.ncbi.nlm.nih.gov/pubmed/35614314
http://dx.doi.org/10.1038/s42003-022-03444-3

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9132957/
https://www.ncbi.nlm.nih.gov/pubmed/35614314
http://dx.doi.org/10.1038/s42003-022-03444-3

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