Development of a mitochondrial sirtuin 4 FRET assay based on its activity for removing 3-hydroxy-3-methylglutaryl (HMG) modification

SIRT4 belongs to one of three mitochondrial sirtuins, which plays important roles in regulating many biological processes and has significant implications for treating several human diseases. However, the development of those small compounds that can modulate SIRT4 activities is limited because ther...

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Autores principales: Li, Yan, Zhao, Yonglong, Cao, Zhuoxian, Wang, Jie, Liu, Ting, Li, Yongjun, Wang, Yuanyuan, He, Bin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2021
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9133976/
https://www.ncbi.nlm.nih.gov/pubmed/35747080
http://dx.doi.org/10.1039/d0ra09424b
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author Li, Yan
Zhao, Yonglong
Cao, Zhuoxian
Wang, Jie
Liu, Ting
Li, Yongjun
Wang, Yuanyuan
He, Bin
author_facet Li, Yan
Zhao, Yonglong
Cao, Zhuoxian
Wang, Jie
Liu, Ting
Li, Yongjun
Wang, Yuanyuan
He, Bin
author_sort Li, Yan
collection PubMed
description SIRT4 belongs to one of three mitochondrial sirtuins, which plays important roles in regulating many biological processes and has significant implications for treating several human diseases. However, the development of those small compounds that can modulate SIRT4 activities is limited because there is no efficient SIRT4 assay available for screening its modulators. Thanks to recent discoveries of several enzymatic activities and substrates for SIRT4, we have developed a FRET-based assay suitable for screening SIRT4 modulators based on its activity of removing 3-hydroxy-3-methylglutaryl (HMG) lysine modification, which could be further coupled with a secondary FRET-based assay for other sirtuins to identify SIRT4-specific inhibitors or activators.
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spelling pubmed-91339762022-06-22 Development of a mitochondrial sirtuin 4 FRET assay based on its activity for removing 3-hydroxy-3-methylglutaryl (HMG) modification Li, Yan Zhao, Yonglong Cao, Zhuoxian Wang, Jie Liu, Ting Li, Yongjun Wang, Yuanyuan He, Bin RSC Adv Chemistry SIRT4 belongs to one of three mitochondrial sirtuins, which plays important roles in regulating many biological processes and has significant implications for treating several human diseases. However, the development of those small compounds that can modulate SIRT4 activities is limited because there is no efficient SIRT4 assay available for screening its modulators. Thanks to recent discoveries of several enzymatic activities and substrates for SIRT4, we have developed a FRET-based assay suitable for screening SIRT4 modulators based on its activity of removing 3-hydroxy-3-methylglutaryl (HMG) lysine modification, which could be further coupled with a secondary FRET-based assay for other sirtuins to identify SIRT4-specific inhibitors or activators. The Royal Society of Chemistry 2021-01-13 /pmc/articles/PMC9133976/ /pubmed/35747080 http://dx.doi.org/10.1039/d0ra09424b Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Li, Yan
Zhao, Yonglong
Cao, Zhuoxian
Wang, Jie
Liu, Ting
Li, Yongjun
Wang, Yuanyuan
He, Bin
Development of a mitochondrial sirtuin 4 FRET assay based on its activity for removing 3-hydroxy-3-methylglutaryl (HMG) modification
title Development of a mitochondrial sirtuin 4 FRET assay based on its activity for removing 3-hydroxy-3-methylglutaryl (HMG) modification
title_full Development of a mitochondrial sirtuin 4 FRET assay based on its activity for removing 3-hydroxy-3-methylglutaryl (HMG) modification
title_fullStr Development of a mitochondrial sirtuin 4 FRET assay based on its activity for removing 3-hydroxy-3-methylglutaryl (HMG) modification
title_full_unstemmed Development of a mitochondrial sirtuin 4 FRET assay based on its activity for removing 3-hydroxy-3-methylglutaryl (HMG) modification
title_short Development of a mitochondrial sirtuin 4 FRET assay based on its activity for removing 3-hydroxy-3-methylglutaryl (HMG) modification
title_sort development of a mitochondrial sirtuin 4 fret assay based on its activity for removing 3-hydroxy-3-methylglutaryl (hmg) modification
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9133976/
https://www.ncbi.nlm.nih.gov/pubmed/35747080
http://dx.doi.org/10.1039/d0ra09424b
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