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An RNA-immunoprecipitation protocol to identify RNAs associated with RNA-binding proteins in cytoplasmic and nuclear Drosophila head fractions

RNA-binding proteins (RBPs) are multifunctional proteins that shuttle between the nucleus and the cytoplasm where they assemble with target RNAs to form multi-molecular complexes. Here, we describe a protocol to selectively identify RNAs associated with RBPs of interest in the cytoplasmic and nuclea...

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Detalles Bibliográficos
Autores principales: Heim, Marjorie, Blot, Lauren, Besse, Florence
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9136351/
https://www.ncbi.nlm.nih.gov/pubmed/35634357
http://dx.doi.org/10.1016/j.xpro.2022.101415