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In vivo gain-of-function cDNA library screening for colonization genes in a mouse model of pulmonary metastasis
We provide a protocol for gain-of-function (GOF) cDNA screen of genes that foster cancer cell colonization of secondary tissues, the last and most lethal step of the metastasis cascade. We present techniques for cDNA viral library preparation and delivery leading up to the recovery of colonization-p...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9136352/ https://www.ncbi.nlm.nih.gov/pubmed/35634360 http://dx.doi.org/10.1016/j.xpro.2022.101413 |