Murine brain tumor microenvironment immunophenotyping using mass cytometry

Here, we present a mass cytometry protocol optimized to examine the phenotype of immune cells within the mouse glioma microenvironment, using a Sleeping Beauty transposon-mediated mouse glioma model. We describe antibody conjugation and titrations for analysis of immune cells. We then detail mouse b...

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Detalles Bibliográficos
Autores principales: McClellan, Brandon L., Alghamri, Mahmoud S., Thalla, Rohit, Lowenstein, Pedro R., Castro, Maria G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9136353/
https://www.ncbi.nlm.nih.gov/pubmed/35634359
http://dx.doi.org/10.1016/j.xpro.2022.101357

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9136353/
https://www.ncbi.nlm.nih.gov/pubmed/35634359
http://dx.doi.org/10.1016/j.xpro.2022.101357

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