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Murine brain tumor microenvironment immunophenotyping using mass cytometry

Here, we present a mass cytometry protocol optimized to examine the phenotype of immune cells within the mouse glioma microenvironment, using a Sleeping Beauty transposon-mediated mouse glioma model. We describe antibody conjugation and titrations for analysis of immune cells. We then detail mouse b...

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Detalles Bibliográficos
Autores principales:	McClellan, Brandon L., Alghamri, Mahmoud S., Thalla, Rohit, Lowenstein, Pedro R., Castro, Maria G.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2022
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9136353/ https://www.ncbi.nlm.nih.gov/pubmed/35634359 http://dx.doi.org/10.1016/j.xpro.2022.101357

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