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Lipogenesis mediated by OGR1 regulates metabolic adaptation to acid stress in cancer cells via autophagy

Malignant tumors exhibit altered metabolism resulting in a highly acidic extracellular microenvironment. Here, we show that cytoplasmic lipid droplet (LD) accumulation, indicative of a lipogenic phenotype, is a cellular adaption to extracellular acidity. LD marker PLIN2 is strongly associated with p...

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Autores principales: Pillai, Smitha, Mahmud, Iqbal, Mahar, Rohit, Griffith, Crystal, Langsen, Michael, Nguyen, Jonathan, Wojtkowiak, Jonathan W., Swietach, Pawel, Gatenby, Robert A., Bui, Marilyn M., Merritt, Matthew E., McDonald, Patricia, Garrett, Timothy J., Gillies, Robert J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9137419/
https://www.ncbi.nlm.nih.gov/pubmed/35545051
http://dx.doi.org/10.1016/j.celrep.2022.110796
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author Pillai, Smitha
Mahmud, Iqbal
Mahar, Rohit
Griffith, Crystal
Langsen, Michael
Nguyen, Jonathan
Wojtkowiak, Jonathan W.
Swietach, Pawel
Gatenby, Robert A.
Bui, Marilyn M.
Merritt, Matthew E.
McDonald, Patricia
Garrett, Timothy J.
Gillies, Robert J.
author_facet Pillai, Smitha
Mahmud, Iqbal
Mahar, Rohit
Griffith, Crystal
Langsen, Michael
Nguyen, Jonathan
Wojtkowiak, Jonathan W.
Swietach, Pawel
Gatenby, Robert A.
Bui, Marilyn M.
Merritt, Matthew E.
McDonald, Patricia
Garrett, Timothy J.
Gillies, Robert J.
author_sort Pillai, Smitha
collection PubMed
description Malignant tumors exhibit altered metabolism resulting in a highly acidic extracellular microenvironment. Here, we show that cytoplasmic lipid droplet (LD) accumulation, indicative of a lipogenic phenotype, is a cellular adaption to extracellular acidity. LD marker PLIN2 is strongly associated with poor overall survival in breast cancer patients. Acid-induced LD accumulation is triggered by activation of the acid-sensing G-protein-coupled receptor (GPCR) OGR1, which is expressed highly in breast tumors. OGR1 depletion inhibits acid-induced lipid accumulation, while activation by a synthetic agonist triggers LD formation. Inhibition of OGR1 downstream signaling abrogates the lipogenic phenotype, which can be rescued with OGR1 ectopic expression. OGR1-depleted cells show growth inhibition under acidic growth conditions in vitro and tumor formation in vivo. Isotope tracing shows that the source of lipid precursors is primarily autophagy-derived ketogenic amino acids. OGR1-depleted cells are defective in endoplasmic reticulum stress response and autophagy and hence fail to accumulate LDs affecting survival under acidic stress.
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spelling pubmed-91374192022-05-27 Lipogenesis mediated by OGR1 regulates metabolic adaptation to acid stress in cancer cells via autophagy Pillai, Smitha Mahmud, Iqbal Mahar, Rohit Griffith, Crystal Langsen, Michael Nguyen, Jonathan Wojtkowiak, Jonathan W. Swietach, Pawel Gatenby, Robert A. Bui, Marilyn M. Merritt, Matthew E. McDonald, Patricia Garrett, Timothy J. Gillies, Robert J. Cell Rep Article Malignant tumors exhibit altered metabolism resulting in a highly acidic extracellular microenvironment. Here, we show that cytoplasmic lipid droplet (LD) accumulation, indicative of a lipogenic phenotype, is a cellular adaption to extracellular acidity. LD marker PLIN2 is strongly associated with poor overall survival in breast cancer patients. Acid-induced LD accumulation is triggered by activation of the acid-sensing G-protein-coupled receptor (GPCR) OGR1, which is expressed highly in breast tumors. OGR1 depletion inhibits acid-induced lipid accumulation, while activation by a synthetic agonist triggers LD formation. Inhibition of OGR1 downstream signaling abrogates the lipogenic phenotype, which can be rescued with OGR1 ectopic expression. OGR1-depleted cells show growth inhibition under acidic growth conditions in vitro and tumor formation in vivo. Isotope tracing shows that the source of lipid precursors is primarily autophagy-derived ketogenic amino acids. OGR1-depleted cells are defective in endoplasmic reticulum stress response and autophagy and hence fail to accumulate LDs affecting survival under acidic stress. 2022-05-10 /pmc/articles/PMC9137419/ /pubmed/35545051 http://dx.doi.org/10.1016/j.celrep.2022.110796 Text en https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ).
spellingShingle Article
Pillai, Smitha
Mahmud, Iqbal
Mahar, Rohit
Griffith, Crystal
Langsen, Michael
Nguyen, Jonathan
Wojtkowiak, Jonathan W.
Swietach, Pawel
Gatenby, Robert A.
Bui, Marilyn M.
Merritt, Matthew E.
McDonald, Patricia
Garrett, Timothy J.
Gillies, Robert J.
Lipogenesis mediated by OGR1 regulates metabolic adaptation to acid stress in cancer cells via autophagy
title Lipogenesis mediated by OGR1 regulates metabolic adaptation to acid stress in cancer cells via autophagy
title_full Lipogenesis mediated by OGR1 regulates metabolic adaptation to acid stress in cancer cells via autophagy
title_fullStr Lipogenesis mediated by OGR1 regulates metabolic adaptation to acid stress in cancer cells via autophagy
title_full_unstemmed Lipogenesis mediated by OGR1 regulates metabolic adaptation to acid stress in cancer cells via autophagy
title_short Lipogenesis mediated by OGR1 regulates metabolic adaptation to acid stress in cancer cells via autophagy
title_sort lipogenesis mediated by ogr1 regulates metabolic adaptation to acid stress in cancer cells via autophagy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9137419/
https://www.ncbi.nlm.nih.gov/pubmed/35545051
http://dx.doi.org/10.1016/j.celrep.2022.110796
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