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The Use of Bacteriophages in Biotechnology and Recent Insights into Proteomics
Phages have certain features, such as their ability to form protein–protein interactions, that make them good candidates for use in a variety of beneficial applications, such as in human or animal health, industry, food science, food safety, and agriculture. It is essential to identify and character...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9137636/ https://www.ncbi.nlm.nih.gov/pubmed/35625297 http://dx.doi.org/10.3390/antibiotics11050653 |
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author | Abril, Ana G. Carrera, Mónica Notario, Vicente Sánchez-Pérez, Ángeles Villa, Tomás G. |
author_facet | Abril, Ana G. Carrera, Mónica Notario, Vicente Sánchez-Pérez, Ángeles Villa, Tomás G. |
author_sort | Abril, Ana G. |
collection | PubMed |
description | Phages have certain features, such as their ability to form protein–protein interactions, that make them good candidates for use in a variety of beneficial applications, such as in human or animal health, industry, food science, food safety, and agriculture. It is essential to identify and characterize the proteins produced by particular phages in order to use these viruses in a variety of functional processes, such as bacterial detection, as vehicles for drug delivery, in vaccine development, and to combat multidrug resistant bacterial infections. Furthermore, phages can also play a major role in the design of a variety of cheap and stable sensors as well as in diagnostic assays that can either specifically identify specific compounds or detect bacteria. This article reviews recently developed phage-based techniques, such as the use of recombinant tempered phages, phage display and phage amplification-based detection. It also encompasses the application of phages as capture elements, biosensors and bioreceptors, with a special emphasis on novel bacteriophage-based mass spectrometry (MS) applications. |
format | Online Article Text |
id | pubmed-9137636 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-91376362022-05-28 The Use of Bacteriophages in Biotechnology and Recent Insights into Proteomics Abril, Ana G. Carrera, Mónica Notario, Vicente Sánchez-Pérez, Ángeles Villa, Tomás G. Antibiotics (Basel) Review Phages have certain features, such as their ability to form protein–protein interactions, that make them good candidates for use in a variety of beneficial applications, such as in human or animal health, industry, food science, food safety, and agriculture. It is essential to identify and characterize the proteins produced by particular phages in order to use these viruses in a variety of functional processes, such as bacterial detection, as vehicles for drug delivery, in vaccine development, and to combat multidrug resistant bacterial infections. Furthermore, phages can also play a major role in the design of a variety of cheap and stable sensors as well as in diagnostic assays that can either specifically identify specific compounds or detect bacteria. This article reviews recently developed phage-based techniques, such as the use of recombinant tempered phages, phage display and phage amplification-based detection. It also encompasses the application of phages as capture elements, biosensors and bioreceptors, with a special emphasis on novel bacteriophage-based mass spectrometry (MS) applications. MDPI 2022-05-13 /pmc/articles/PMC9137636/ /pubmed/35625297 http://dx.doi.org/10.3390/antibiotics11050653 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review Abril, Ana G. Carrera, Mónica Notario, Vicente Sánchez-Pérez, Ángeles Villa, Tomás G. The Use of Bacteriophages in Biotechnology and Recent Insights into Proteomics |
title | The Use of Bacteriophages in Biotechnology and Recent Insights into Proteomics |
title_full | The Use of Bacteriophages in Biotechnology and Recent Insights into Proteomics |
title_fullStr | The Use of Bacteriophages in Biotechnology and Recent Insights into Proteomics |
title_full_unstemmed | The Use of Bacteriophages in Biotechnology and Recent Insights into Proteomics |
title_short | The Use of Bacteriophages in Biotechnology and Recent Insights into Proteomics |
title_sort | use of bacteriophages in biotechnology and recent insights into proteomics |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9137636/ https://www.ncbi.nlm.nih.gov/pubmed/35625297 http://dx.doi.org/10.3390/antibiotics11050653 |
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