Cargando…
Identification of Modulators of the C. elegans Aryl Hydrocarbon Receptor and Characterization of Transcriptomic and Metabolic AhR-1 Profiles
The Aryl hydrocarbon Receptor (AhR) is a xenobiotic sensor in vertebrates, regulating the metabolism of its own ligands. However, no ligand has been identified to date for any AhR in invertebrates. In C. elegans, the AhR ortholog, AHR-1, displays physiological functions. Therefore, we compared the t...
Autores principales: | , , , , , , , , , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9137885/ https://www.ncbi.nlm.nih.gov/pubmed/35624894 http://dx.doi.org/10.3390/antiox11051030 |
_version_ | 1784714489748258816 |
---|---|
author | Larigot, Lucie Bui, Linh-Chi de Bouvier, Marine Pierre, Ophélie Pinon, Grégory Fiocca, Justine Ozeir, Mohammad Tourette, Cendrine Ottolenghi, Chris Imbeaud, Sandrine Pontoizeau, Clément Blaise, Benjamin J. Chevallier, Aline Tomkiewicz, Céline Legrand, Béatrice Elena-Herrmann, Bénédicte Néri, Christian Brinkmann, Vanessa Nioche, Pierre Barouki, Robert Ventura, Natascia Dairou, Julien Coumoul, Xavier |
author_facet | Larigot, Lucie Bui, Linh-Chi de Bouvier, Marine Pierre, Ophélie Pinon, Grégory Fiocca, Justine Ozeir, Mohammad Tourette, Cendrine Ottolenghi, Chris Imbeaud, Sandrine Pontoizeau, Clément Blaise, Benjamin J. Chevallier, Aline Tomkiewicz, Céline Legrand, Béatrice Elena-Herrmann, Bénédicte Néri, Christian Brinkmann, Vanessa Nioche, Pierre Barouki, Robert Ventura, Natascia Dairou, Julien Coumoul, Xavier |
author_sort | Larigot, Lucie |
collection | PubMed |
description | The Aryl hydrocarbon Receptor (AhR) is a xenobiotic sensor in vertebrates, regulating the metabolism of its own ligands. However, no ligand has been identified to date for any AhR in invertebrates. In C. elegans, the AhR ortholog, AHR-1, displays physiological functions. Therefore, we compared the transcriptomic and metabolic profiles of worms expressing AHR-1 or not and investigated the putative panel of chemical AHR-1 modulators. The metabolomic profiling indicated a role for AHR-1 in amino acids, carbohydrates, and fatty acids metabolism. The transcriptional profiling in neurons expressing AHR-1, identified 95 down-regulated genes and 76 up-regulated genes associated with neuronal and metabolic functions in the nervous system. A gene reporter system allowed us to identify several AHR-1 modulators including bacterial, dietary, or environmental compounds. These results shed new light on the biological functions of AHR-1 in C. elegans and perspectives on the evolution of the AhR functions across species. |
format | Online Article Text |
id | pubmed-9137885 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-91378852022-05-28 Identification of Modulators of the C. elegans Aryl Hydrocarbon Receptor and Characterization of Transcriptomic and Metabolic AhR-1 Profiles Larigot, Lucie Bui, Linh-Chi de Bouvier, Marine Pierre, Ophélie Pinon, Grégory Fiocca, Justine Ozeir, Mohammad Tourette, Cendrine Ottolenghi, Chris Imbeaud, Sandrine Pontoizeau, Clément Blaise, Benjamin J. Chevallier, Aline Tomkiewicz, Céline Legrand, Béatrice Elena-Herrmann, Bénédicte Néri, Christian Brinkmann, Vanessa Nioche, Pierre Barouki, Robert Ventura, Natascia Dairou, Julien Coumoul, Xavier Antioxidants (Basel) Article The Aryl hydrocarbon Receptor (AhR) is a xenobiotic sensor in vertebrates, regulating the metabolism of its own ligands. However, no ligand has been identified to date for any AhR in invertebrates. In C. elegans, the AhR ortholog, AHR-1, displays physiological functions. Therefore, we compared the transcriptomic and metabolic profiles of worms expressing AHR-1 or not and investigated the putative panel of chemical AHR-1 modulators. The metabolomic profiling indicated a role for AHR-1 in amino acids, carbohydrates, and fatty acids metabolism. The transcriptional profiling in neurons expressing AHR-1, identified 95 down-regulated genes and 76 up-regulated genes associated with neuronal and metabolic functions in the nervous system. A gene reporter system allowed us to identify several AHR-1 modulators including bacterial, dietary, or environmental compounds. These results shed new light on the biological functions of AHR-1 in C. elegans and perspectives on the evolution of the AhR functions across species. MDPI 2022-05-23 /pmc/articles/PMC9137885/ /pubmed/35624894 http://dx.doi.org/10.3390/antiox11051030 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Larigot, Lucie Bui, Linh-Chi de Bouvier, Marine Pierre, Ophélie Pinon, Grégory Fiocca, Justine Ozeir, Mohammad Tourette, Cendrine Ottolenghi, Chris Imbeaud, Sandrine Pontoizeau, Clément Blaise, Benjamin J. Chevallier, Aline Tomkiewicz, Céline Legrand, Béatrice Elena-Herrmann, Bénédicte Néri, Christian Brinkmann, Vanessa Nioche, Pierre Barouki, Robert Ventura, Natascia Dairou, Julien Coumoul, Xavier Identification of Modulators of the C. elegans Aryl Hydrocarbon Receptor and Characterization of Transcriptomic and Metabolic AhR-1 Profiles |
title | Identification of Modulators of the C. elegans Aryl Hydrocarbon Receptor and Characterization of Transcriptomic and Metabolic AhR-1 Profiles |
title_full | Identification of Modulators of the C. elegans Aryl Hydrocarbon Receptor and Characterization of Transcriptomic and Metabolic AhR-1 Profiles |
title_fullStr | Identification of Modulators of the C. elegans Aryl Hydrocarbon Receptor and Characterization of Transcriptomic and Metabolic AhR-1 Profiles |
title_full_unstemmed | Identification of Modulators of the C. elegans Aryl Hydrocarbon Receptor and Characterization of Transcriptomic and Metabolic AhR-1 Profiles |
title_short | Identification of Modulators of the C. elegans Aryl Hydrocarbon Receptor and Characterization of Transcriptomic and Metabolic AhR-1 Profiles |
title_sort | identification of modulators of the c. elegans aryl hydrocarbon receptor and characterization of transcriptomic and metabolic ahr-1 profiles |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9137885/ https://www.ncbi.nlm.nih.gov/pubmed/35624894 http://dx.doi.org/10.3390/antiox11051030 |
work_keys_str_mv | AT larigotlucie identificationofmodulatorsofthecelegansarylhydrocarbonreceptorandcharacterizationoftranscriptomicandmetabolicahr1profiles AT builinhchi identificationofmodulatorsofthecelegansarylhydrocarbonreceptorandcharacterizationoftranscriptomicandmetabolicahr1profiles AT debouviermarine identificationofmodulatorsofthecelegansarylhydrocarbonreceptorandcharacterizationoftranscriptomicandmetabolicahr1profiles AT pierreophelie identificationofmodulatorsofthecelegansarylhydrocarbonreceptorandcharacterizationoftranscriptomicandmetabolicahr1profiles AT pinongregory identificationofmodulatorsofthecelegansarylhydrocarbonreceptorandcharacterizationoftranscriptomicandmetabolicahr1profiles AT fioccajustine identificationofmodulatorsofthecelegansarylhydrocarbonreceptorandcharacterizationoftranscriptomicandmetabolicahr1profiles AT ozeirmohammad identificationofmodulatorsofthecelegansarylhydrocarbonreceptorandcharacterizationoftranscriptomicandmetabolicahr1profiles AT tourettecendrine identificationofmodulatorsofthecelegansarylhydrocarbonreceptorandcharacterizationoftranscriptomicandmetabolicahr1profiles AT ottolenghichris identificationofmodulatorsofthecelegansarylhydrocarbonreceptorandcharacterizationoftranscriptomicandmetabolicahr1profiles AT imbeaudsandrine identificationofmodulatorsofthecelegansarylhydrocarbonreceptorandcharacterizationoftranscriptomicandmetabolicahr1profiles AT pontoizeauclement identificationofmodulatorsofthecelegansarylhydrocarbonreceptorandcharacterizationoftranscriptomicandmetabolicahr1profiles AT blaisebenjaminj identificationofmodulatorsofthecelegansarylhydrocarbonreceptorandcharacterizationoftranscriptomicandmetabolicahr1profiles AT chevallieraline identificationofmodulatorsofthecelegansarylhydrocarbonreceptorandcharacterizationoftranscriptomicandmetabolicahr1profiles AT tomkiewiczceline identificationofmodulatorsofthecelegansarylhydrocarbonreceptorandcharacterizationoftranscriptomicandmetabolicahr1profiles AT legrandbeatrice identificationofmodulatorsofthecelegansarylhydrocarbonreceptorandcharacterizationoftranscriptomicandmetabolicahr1profiles AT elenaherrmannbenedicte identificationofmodulatorsofthecelegansarylhydrocarbonreceptorandcharacterizationoftranscriptomicandmetabolicahr1profiles AT nerichristian identificationofmodulatorsofthecelegansarylhydrocarbonreceptorandcharacterizationoftranscriptomicandmetabolicahr1profiles AT brinkmannvanessa identificationofmodulatorsofthecelegansarylhydrocarbonreceptorandcharacterizationoftranscriptomicandmetabolicahr1profiles AT niochepierre identificationofmodulatorsofthecelegansarylhydrocarbonreceptorandcharacterizationoftranscriptomicandmetabolicahr1profiles AT baroukirobert identificationofmodulatorsofthecelegansarylhydrocarbonreceptorandcharacterizationoftranscriptomicandmetabolicahr1profiles AT venturanatascia identificationofmodulatorsofthecelegansarylhydrocarbonreceptorandcharacterizationoftranscriptomicandmetabolicahr1profiles AT dairoujulien identificationofmodulatorsofthecelegansarylhydrocarbonreceptorandcharacterizationoftranscriptomicandmetabolicahr1profiles AT coumoulxavier identificationofmodulatorsofthecelegansarylhydrocarbonreceptorandcharacterizationoftranscriptomicandmetabolicahr1profiles |