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Comparing and Optimizing RNA Extraction from the Pancreas of Diabetic and Healthy Rats for Gene Expression Analyses

Advanced differential gene expression analysis requires high-quality RNA. However, isolating intact pancreatic RNA is challenging due to abundant pancreatic ribonucleases, which limits efficient downstream gene expression analysis. RNAlater treatment reduces endogenous ribonucleases effects through...

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Detalles Bibliográficos
Autores principales: Al-Adsani, Amani M., Barhoush, Sahar A., Bastaki, Nasmah K., Al-Bustan, Suzanne A., Al-Qattan, Khaled K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9141713/
https://www.ncbi.nlm.nih.gov/pubmed/35627265
http://dx.doi.org/10.3390/genes13050881