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Automated vitrification of cryo-EM samples with controllable sample thickness using suction and real-time optical inspection

The speed and efficiency of data collection and image processing in cryo-electron microscopy have increased over the last decade. However, cryo specimen preparation techniques have lagged and faster, more reproducible specimen preparation devices are needed. Here, we present a vitrification device w...

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Detalles Bibliográficos
Autores principales: Koning, Roman I., Vader, Hildo, van Nugteren, Martijn, Grocutt, Peter A., Yang, Wen, Renault, Ludovic L. R., Koster, Abraham J., Kamp, Arnold C. F., Schwertner, Michael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9142589/
https://www.ncbi.nlm.nih.gov/pubmed/35624105
http://dx.doi.org/10.1038/s41467-022-30562-7