An Improved Method for Quantification of Viable Fusarium Cells in Infected Soil Products by Propidium Monoazide Coupled with Real-Time PCR

Fusarium is a soil-borne pathogen that causes root rot disease in cucumber. To date, quantitative real-time PCR (qPCR) is a common tool to detect the content of Fusarium in soil. However, qPCR cannot distinguish between viable and nonviable cells. The aim of this study was to develop a detection tec...

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Detalles Bibliográficos
Autores principales: Chen, Lida, Li, Lei, Xie, Xuewen, Chai, Ali, Shi, Yanxia, Fan, Tengfei, Xie, Jianming, Li, Baoju
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9143521/
https://www.ncbi.nlm.nih.gov/pubmed/35630479
http://dx.doi.org/10.3390/microorganisms10051037

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9143521/
https://www.ncbi.nlm.nih.gov/pubmed/35630479
http://dx.doi.org/10.3390/microorganisms10051037

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