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Applying EDTA in Chelating Excess Metal Ions to Improve Downstream DNA Recovery from Mine Tailings for Long-Read Amplicon Sequencing of Acidophilic Fungi Communities

The hostile environment of mine tailings contains unique microbial life capable of bioleaching. The metagenomic analysis of such an environment provides an in-depth understanding of the microbial life and its potential, especially in biomining operations. However, DNA recovery from samples collected...

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Detalles Bibliográficos
Autores principales: Nkuna, Rosina, Ijoma, Grace N., Matambo, Tonderayi S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9143545/
https://www.ncbi.nlm.nih.gov/pubmed/35628675
http://dx.doi.org/10.3390/jof8050419