Spontaneous DNA Synapsis by Forming Noncanonical Intermolecular Structures

We report the spontaneous formation of DNA-DNA junctions in solution in the absence of proteins visualised using atomic force microscopy. The synapsis position fits with potential G-quadruplex (G4) sites. In contrast to the Holliday structure, these conjugates have an affinity for G4 antibodies. Mol...

Descripción completa

Detalles Bibliográficos
Autores principales: Severov, Viacheslav, Tsvetkov, Vladimir, Barinov, Nikolay, Babenko, Vladislav, Klinov, Dmitry, Pozmogova, Galina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9144187/
https://www.ncbi.nlm.nih.gov/pubmed/35632001
http://dx.doi.org/10.3390/polym14102118
_version_ 1784715987562528768
author Severov, Viacheslav
Tsvetkov, Vladimir
Barinov, Nikolay
Babenko, Vladislav
Klinov, Dmitry
Pozmogova, Galina
author_facet Severov, Viacheslav
Tsvetkov, Vladimir
Barinov, Nikolay
Babenko, Vladislav
Klinov, Dmitry
Pozmogova, Galina
author_sort Severov, Viacheslav
collection PubMed
description We report the spontaneous formation of DNA-DNA junctions in solution in the absence of proteins visualised using atomic force microscopy. The synapsis position fits with potential G-quadruplex (G4) sites. In contrast to the Holliday structure, these conjugates have an affinity for G4 antibodies. Molecular modelling was used to elucidate the possible G4/IM-synaptic complex structures. Our results indicate a new role of the intermolecular noncanonical structures in chromatin architecture and genomic rearrangement.
format Online
Article
Text
id pubmed-9144187
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-91441872022-05-29 Spontaneous DNA Synapsis by Forming Noncanonical Intermolecular Structures Severov, Viacheslav Tsvetkov, Vladimir Barinov, Nikolay Babenko, Vladislav Klinov, Dmitry Pozmogova, Galina Polymers (Basel) Article We report the spontaneous formation of DNA-DNA junctions in solution in the absence of proteins visualised using atomic force microscopy. The synapsis position fits with potential G-quadruplex (G4) sites. In contrast to the Holliday structure, these conjugates have an affinity for G4 antibodies. Molecular modelling was used to elucidate the possible G4/IM-synaptic complex structures. Our results indicate a new role of the intermolecular noncanonical structures in chromatin architecture and genomic rearrangement. MDPI 2022-05-23 /pmc/articles/PMC9144187/ /pubmed/35632001 http://dx.doi.org/10.3390/polym14102118 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Severov, Viacheslav
Tsvetkov, Vladimir
Barinov, Nikolay
Babenko, Vladislav
Klinov, Dmitry
Pozmogova, Galina
Spontaneous DNA Synapsis by Forming Noncanonical Intermolecular Structures
title Spontaneous DNA Synapsis by Forming Noncanonical Intermolecular Structures
title_full Spontaneous DNA Synapsis by Forming Noncanonical Intermolecular Structures
title_fullStr Spontaneous DNA Synapsis by Forming Noncanonical Intermolecular Structures
title_full_unstemmed Spontaneous DNA Synapsis by Forming Noncanonical Intermolecular Structures
title_short Spontaneous DNA Synapsis by Forming Noncanonical Intermolecular Structures
title_sort spontaneous dna synapsis by forming noncanonical intermolecular structures
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9144187/
https://www.ncbi.nlm.nih.gov/pubmed/35632001
http://dx.doi.org/10.3390/polym14102118
work_keys_str_mv AT severovviacheslav spontaneousdnasynapsisbyformingnoncanonicalintermolecularstructures
AT tsvetkovvladimir spontaneousdnasynapsisbyformingnoncanonicalintermolecularstructures
AT barinovnikolay spontaneousdnasynapsisbyformingnoncanonicalintermolecularstructures
AT babenkovladislav spontaneousdnasynapsisbyformingnoncanonicalintermolecularstructures
AT klinovdmitry spontaneousdnasynapsisbyformingnoncanonicalintermolecularstructures
AT pozmogovagalina spontaneousdnasynapsisbyformingnoncanonicalintermolecularstructures

Ejemplares similares