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Development of a Highly Sensitive Nested PCR and Its Application for the Diagnosis of Cutaneous Leishmaniasis in Sri Lanka

The recent surge in cutaneous leishmaniasis (CL) in Sri Lanka has rendered clinical diagnosis difficult; thus, laboratory confirmation is indispensable. A modified (two novel inner primers to detect CL caused by Leishmania donovani) nested Internal Transcribed Spacer-1 (ITS1) PCR-Restriction Fragmen...

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Detalles Bibliográficos
Autores principales: De Silva, Nirmitha Lalindi, De Silva, Viraji Nefertiti Hiromel, Deerasinghe, Arachchige Theja Hemapala, Rathnapala, Upeksha Lakmini, Itoh, Makoto, Takagi, Hidekazu, Weerasooriya, Mirani Vasanthamala, Kato, Hirotomo, Yahathugoda, Thishan Channa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9145043/
https://www.ncbi.nlm.nih.gov/pubmed/35630433
http://dx.doi.org/10.3390/microorganisms10050990