Are Hamsters a Suitable Model for Evaluating the Immunogenicity of RBD-Based Anti-COVID-19 Subunit Vaccines?

Currently, SARS-CoV-2 spike receptor-binding-domain (RBD)-based vaccines are considered one of the most effective weapons against COVID-19. During the first step of assessing vaccine immunogenicity, a mouse model is often used. In this paper, we tested the use of five experimental animals (mice, ham...

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Autores principales: Merkuleva, Iuliia A., Shcherbakov, Dmitry N., Borgoyakova, Mariya B., Isaeva, Anastasiya A., Nesmeyanova, Valentina S., Volkova, Natalia V., Aripov, Vazirbek S., Shanshin, Daniil V., Karpenko, Larisa I., Belenkaya, Svetlana V., Kazachinskaia, Elena I., Volosnikova, Ekaterina A., Esina, Tatiana I., Sergeev, Alexandr A., Titova, Kseniia A., Konyakhina, Yulia V., Zaykovskaya, Anna V., Pyankov, Oleg V., Kolosova, Evgeniia A., Viktorina, Olesya E., Shelemba, Arseniya A., Rudometov, Andrey P., Ilyichev, Alexander A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9146860/
https://www.ncbi.nlm.nih.gov/pubmed/35632800
http://dx.doi.org/10.3390/v14051060
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author Merkuleva, Iuliia A.
Shcherbakov, Dmitry N.
Borgoyakova, Mariya B.
Isaeva, Anastasiya A.
Nesmeyanova, Valentina S.
Volkova, Natalia V.
Aripov, Vazirbek S.
Shanshin, Daniil V.
Karpenko, Larisa I.
Belenkaya, Svetlana V.
Kazachinskaia, Elena I.
Volosnikova, Ekaterina A.
Esina, Tatiana I.
Sergeev, Alexandr A.
Titova, Kseniia A.
Konyakhina, Yulia V.
Zaykovskaya, Anna V.
Pyankov, Oleg V.
Kolosova, Evgeniia A.
Viktorina, Olesya E.
Shelemba, Arseniya A.
Rudometov, Andrey P.
Ilyichev, Alexander A.
author_facet Merkuleva, Iuliia A.
Shcherbakov, Dmitry N.
Borgoyakova, Mariya B.
Isaeva, Anastasiya A.
Nesmeyanova, Valentina S.
Volkova, Natalia V.
Aripov, Vazirbek S.
Shanshin, Daniil V.
Karpenko, Larisa I.
Belenkaya, Svetlana V.
Kazachinskaia, Elena I.
Volosnikova, Ekaterina A.
Esina, Tatiana I.
Sergeev, Alexandr A.
Titova, Kseniia A.
Konyakhina, Yulia V.
Zaykovskaya, Anna V.
Pyankov, Oleg V.
Kolosova, Evgeniia A.
Viktorina, Olesya E.
Shelemba, Arseniya A.
Rudometov, Andrey P.
Ilyichev, Alexander A.
author_sort Merkuleva, Iuliia A.
collection PubMed
description Currently, SARS-CoV-2 spike receptor-binding-domain (RBD)-based vaccines are considered one of the most effective weapons against COVID-19. During the first step of assessing vaccine immunogenicity, a mouse model is often used. In this paper, we tested the use of five experimental animals (mice, hamsters, rabbits, ferrets, and chickens) for RBD immunogenicity assessments. The humoral immune response was evaluated by ELISA and virus-neutralization assays. The data obtained show hamsters to be the least suitable candidates for RBD immunogenicity testing and, hence, assessing the protective efficacy of RBD-based vaccines.
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spelling pubmed-91468602022-05-29 Are Hamsters a Suitable Model for Evaluating the Immunogenicity of RBD-Based Anti-COVID-19 Subunit Vaccines? Merkuleva, Iuliia A. Shcherbakov, Dmitry N. Borgoyakova, Mariya B. Isaeva, Anastasiya A. Nesmeyanova, Valentina S. Volkova, Natalia V. Aripov, Vazirbek S. Shanshin, Daniil V. Karpenko, Larisa I. Belenkaya, Svetlana V. Kazachinskaia, Elena I. Volosnikova, Ekaterina A. Esina, Tatiana I. Sergeev, Alexandr A. Titova, Kseniia A. Konyakhina, Yulia V. Zaykovskaya, Anna V. Pyankov, Oleg V. Kolosova, Evgeniia A. Viktorina, Olesya E. Shelemba, Arseniya A. Rudometov, Andrey P. Ilyichev, Alexander A. Viruses Article Currently, SARS-CoV-2 spike receptor-binding-domain (RBD)-based vaccines are considered one of the most effective weapons against COVID-19. During the first step of assessing vaccine immunogenicity, a mouse model is often used. In this paper, we tested the use of five experimental animals (mice, hamsters, rabbits, ferrets, and chickens) for RBD immunogenicity assessments. The humoral immune response was evaluated by ELISA and virus-neutralization assays. The data obtained show hamsters to be the least suitable candidates for RBD immunogenicity testing and, hence, assessing the protective efficacy of RBD-based vaccines. MDPI 2022-05-16 /pmc/articles/PMC9146860/ /pubmed/35632800 http://dx.doi.org/10.3390/v14051060 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Merkuleva, Iuliia A.
Shcherbakov, Dmitry N.
Borgoyakova, Mariya B.
Isaeva, Anastasiya A.
Nesmeyanova, Valentina S.
Volkova, Natalia V.
Aripov, Vazirbek S.
Shanshin, Daniil V.
Karpenko, Larisa I.
Belenkaya, Svetlana V.
Kazachinskaia, Elena I.
Volosnikova, Ekaterina A.
Esina, Tatiana I.
Sergeev, Alexandr A.
Titova, Kseniia A.
Konyakhina, Yulia V.
Zaykovskaya, Anna V.
Pyankov, Oleg V.
Kolosova, Evgeniia A.
Viktorina, Olesya E.
Shelemba, Arseniya A.
Rudometov, Andrey P.
Ilyichev, Alexander A.
Are Hamsters a Suitable Model for Evaluating the Immunogenicity of RBD-Based Anti-COVID-19 Subunit Vaccines?
title Are Hamsters a Suitable Model for Evaluating the Immunogenicity of RBD-Based Anti-COVID-19 Subunit Vaccines?
title_full Are Hamsters a Suitable Model for Evaluating the Immunogenicity of RBD-Based Anti-COVID-19 Subunit Vaccines?
title_fullStr Are Hamsters a Suitable Model for Evaluating the Immunogenicity of RBD-Based Anti-COVID-19 Subunit Vaccines?
title_full_unstemmed Are Hamsters a Suitable Model for Evaluating the Immunogenicity of RBD-Based Anti-COVID-19 Subunit Vaccines?
title_short Are Hamsters a Suitable Model for Evaluating the Immunogenicity of RBD-Based Anti-COVID-19 Subunit Vaccines?
title_sort are hamsters a suitable model for evaluating the immunogenicity of rbd-based anti-covid-19 subunit vaccines?
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9146860/
https://www.ncbi.nlm.nih.gov/pubmed/35632800
http://dx.doi.org/10.3390/v14051060
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