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Molecular and Pathogenic Characterization of Fusarium Species Associated with Corm Rot Disease in Saffron from China

Saffron (Crocus sativus L.) is a commercial spice crop well-known throughout the world, valued for culinary, colorant, and pharmaceutical purposes. In China, Fusarium nirenbergiae was detected as causative agent of saffron corm rot, the most pervasive disease for the first time in 2020. In the prese...

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Autores principales: Mirghasempour, Seyed Ali, Studholme, David J., Chen, Weiliang, Zhu, Weidong, Mao, Bizeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9147734/
https://www.ncbi.nlm.nih.gov/pubmed/35628770
http://dx.doi.org/10.3390/jof8050515
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author Mirghasempour, Seyed Ali
Studholme, David J.
Chen, Weiliang
Zhu, Weidong
Mao, Bizeng
author_facet Mirghasempour, Seyed Ali
Studholme, David J.
Chen, Weiliang
Zhu, Weidong
Mao, Bizeng
author_sort Mirghasempour, Seyed Ali
collection PubMed
description Saffron (Crocus sativus L.) is a commercial spice crop well-known throughout the world, valued for culinary, colorant, and pharmaceutical purposes. In China, Fusarium nirenbergiae was detected as causative agent of saffron corm rot, the most pervasive disease for the first time in 2020. In the present study, 261 Fusarium-like isolates were recovered from 120 rotted corms in four saffron producing fields at Zhejiang, Shanghai, and Yunnan provinces, China, in 2021. A combination of morpho-cultural features and multilocus sequence analysis (MLSA) of the concatenated rpb2 (DNA-directed RNA polymerase II largest subunit) and tef1 (translation elongation factor 1-α) partial sequences showed that the isolates from saffron belong to Fusarium nirenbergiae as well as F. commune, and F. annulatum with isolation frequencies of 58.2%, 26.8%, and 14.9%, respectively. Notably, F. commune was more prevalent than F. annulatum in the collected samples. Pathogenicity tests confirmed that both species were pathogenic on saffron corm. This is the first report of F. annulatum and F. commune causing corm rot of saffron, globally. Outcomes of the current research demonstrate that Fusarium spp. associated with saffron corm rot are more diverse than previously reported. Furthermore, some plants were infected by two or more Fusarium species. Our findings broaden knowledge about Fusarium spp. that inflict corm rot and assist the development of control measures.
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spelling pubmed-91477342022-05-29 Molecular and Pathogenic Characterization of Fusarium Species Associated with Corm Rot Disease in Saffron from China Mirghasempour, Seyed Ali Studholme, David J. Chen, Weiliang Zhu, Weidong Mao, Bizeng J Fungi (Basel) Article Saffron (Crocus sativus L.) is a commercial spice crop well-known throughout the world, valued for culinary, colorant, and pharmaceutical purposes. In China, Fusarium nirenbergiae was detected as causative agent of saffron corm rot, the most pervasive disease for the first time in 2020. In the present study, 261 Fusarium-like isolates were recovered from 120 rotted corms in four saffron producing fields at Zhejiang, Shanghai, and Yunnan provinces, China, in 2021. A combination of morpho-cultural features and multilocus sequence analysis (MLSA) of the concatenated rpb2 (DNA-directed RNA polymerase II largest subunit) and tef1 (translation elongation factor 1-α) partial sequences showed that the isolates from saffron belong to Fusarium nirenbergiae as well as F. commune, and F. annulatum with isolation frequencies of 58.2%, 26.8%, and 14.9%, respectively. Notably, F. commune was more prevalent than F. annulatum in the collected samples. Pathogenicity tests confirmed that both species were pathogenic on saffron corm. This is the first report of F. annulatum and F. commune causing corm rot of saffron, globally. Outcomes of the current research demonstrate that Fusarium spp. associated with saffron corm rot are more diverse than previously reported. Furthermore, some plants were infected by two or more Fusarium species. Our findings broaden knowledge about Fusarium spp. that inflict corm rot and assist the development of control measures. MDPI 2022-05-17 /pmc/articles/PMC9147734/ /pubmed/35628770 http://dx.doi.org/10.3390/jof8050515 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Mirghasempour, Seyed Ali
Studholme, David J.
Chen, Weiliang
Zhu, Weidong
Mao, Bizeng
Molecular and Pathogenic Characterization of Fusarium Species Associated with Corm Rot Disease in Saffron from China
title Molecular and Pathogenic Characterization of Fusarium Species Associated with Corm Rot Disease in Saffron from China
title_full Molecular and Pathogenic Characterization of Fusarium Species Associated with Corm Rot Disease in Saffron from China
title_fullStr Molecular and Pathogenic Characterization of Fusarium Species Associated with Corm Rot Disease in Saffron from China
title_full_unstemmed Molecular and Pathogenic Characterization of Fusarium Species Associated with Corm Rot Disease in Saffron from China
title_short Molecular and Pathogenic Characterization of Fusarium Species Associated with Corm Rot Disease in Saffron from China
title_sort molecular and pathogenic characterization of fusarium species associated with corm rot disease in saffron from china
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9147734/
https://www.ncbi.nlm.nih.gov/pubmed/35628770
http://dx.doi.org/10.3390/jof8050515
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