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A New Method to Characterize Conformation-Specific Antibody by a Combination of Agarose Native Gel Electrophoresis and Contact Blotting

In this study, we review the agarose native gel electrophoresis that separates proteins and macromolecular complexes in their native state and transfer of the separated proteins from the agarose gel to membranes by contact blotting which retains the native state of these structures. Green fluorescen...

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Detalles Bibliográficos
Autores principales: Akuta, Teruo, Maruyama, Toshiaki, Sakuma, Chiaki, Nakagawa, Masataka, Tomioka, Yui, Entzminger, Kevin, Fleming, Jonathan K., Sato, Ryo, Shibata, Takashi, Kurosawa, Yasunori, Okumura, C. J., Arakawa, Tsutomu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9149980/
https://www.ncbi.nlm.nih.gov/pubmed/35645209
http://dx.doi.org/10.3390/antib11020036