A New Method to Characterize Conformation-Specific Antibody by a Combination of Agarose Native Gel Electrophoresis and Contact Blotting

In this study, we review the agarose native gel electrophoresis that separates proteins and macromolecular complexes in their native state and transfer of the separated proteins from the agarose gel to membranes by contact blotting which retains the native state of these structures. Green fluorescen...

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Autores principales: Akuta, Teruo, Maruyama, Toshiaki, Sakuma, Chiaki, Nakagawa, Masataka, Tomioka, Yui, Entzminger, Kevin, Fleming, Jonathan K., Sato, Ryo, Shibata, Takashi, Kurosawa, Yasunori, Okumura, C. J., Arakawa, Tsutomu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Review
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9149980/
https://www.ncbi.nlm.nih.gov/pubmed/35645209
http://dx.doi.org/10.3390/antib11020036
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author Akuta, Teruo
Maruyama, Toshiaki
Sakuma, Chiaki
Nakagawa, Masataka
Tomioka, Yui
Entzminger, Kevin
Fleming, Jonathan K.
Sato, Ryo
Shibata, Takashi
Kurosawa, Yasunori
Okumura, C. J.
Arakawa, Tsutomu
author_facet Akuta, Teruo
Maruyama, Toshiaki
Sakuma, Chiaki
Nakagawa, Masataka
Tomioka, Yui
Entzminger, Kevin
Fleming, Jonathan K.
Sato, Ryo
Shibata, Takashi
Kurosawa, Yasunori
Okumura, C. J.
Arakawa, Tsutomu
author_sort Akuta, Teruo
collection PubMed
description In this study, we review the agarose native gel electrophoresis that separates proteins and macromolecular complexes in their native state and transfer of the separated proteins from the agarose gel to membranes by contact blotting which retains the native state of these structures. Green fluorescent protein showed functional state both on agarose gel and blotted membrane. Based on the combined procedures, we discovered conformation-specific monoclonal antibodies against PLXDC2 and SARS-CoV-2 spike protein.
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spelling pubmed-91499802022-05-31 A New Method to Characterize Conformation-Specific Antibody by a Combination of Agarose Native Gel Electrophoresis and Contact Blotting Akuta, Teruo Maruyama, Toshiaki Sakuma, Chiaki Nakagawa, Masataka Tomioka, Yui Entzminger, Kevin Fleming, Jonathan K. Sato, Ryo Shibata, Takashi Kurosawa, Yasunori Okumura, C. J. Arakawa, Tsutomu Antibodies (Basel) Review In this study, we review the agarose native gel electrophoresis that separates proteins and macromolecular complexes in their native state and transfer of the separated proteins from the agarose gel to membranes by contact blotting which retains the native state of these structures. Green fluorescent protein showed functional state both on agarose gel and blotted membrane. Based on the combined procedures, we discovered conformation-specific monoclonal antibodies against PLXDC2 and SARS-CoV-2 spike protein. MDPI 2022-05-12 /pmc/articles/PMC9149980/ /pubmed/35645209 http://dx.doi.org/10.3390/antib11020036 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Akuta, Teruo
Maruyama, Toshiaki
Sakuma, Chiaki
Nakagawa, Masataka
Tomioka, Yui
Entzminger, Kevin
Fleming, Jonathan K.
Sato, Ryo
Shibata, Takashi
Kurosawa, Yasunori
Okumura, C. J.
Arakawa, Tsutomu
A New Method to Characterize Conformation-Specific Antibody by a Combination of Agarose Native Gel Electrophoresis and Contact Blotting
title A New Method to Characterize Conformation-Specific Antibody by a Combination of Agarose Native Gel Electrophoresis and Contact Blotting
title_full A New Method to Characterize Conformation-Specific Antibody by a Combination of Agarose Native Gel Electrophoresis and Contact Blotting
title_fullStr A New Method to Characterize Conformation-Specific Antibody by a Combination of Agarose Native Gel Electrophoresis and Contact Blotting
title_full_unstemmed A New Method to Characterize Conformation-Specific Antibody by a Combination of Agarose Native Gel Electrophoresis and Contact Blotting
title_short A New Method to Characterize Conformation-Specific Antibody by a Combination of Agarose Native Gel Electrophoresis and Contact Blotting
title_sort new method to characterize conformation-specific antibody by a combination of agarose native gel electrophoresis and contact blotting
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9149980/
https://www.ncbi.nlm.nih.gov/pubmed/35645209
http://dx.doi.org/10.3390/antib11020036
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