CRISPR/Cas9 gRNA activity depends on free energy changes and on the target PAM context

A major challenge of CRISPR/Cas9-mediated genome engineering is that not all guide RNAs (gRNAs) cleave the DNA efficiently. Although the heterogeneity of gRNA activity is well recognized, the current understanding of how CRISPR/Cas9 activity is regulated remains incomplete. Here, we identify a sweet...

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Detalles Bibliográficos
Autores principales: Corsi, Giulia I., Qu, Kunli, Alkan, Ferhat, Pan, Xiaoguang, Luo, Yonglun, Gorodkin, Jan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9151727/
https://www.ncbi.nlm.nih.gov/pubmed/35637227
http://dx.doi.org/10.1038/s41467-022-30515-0

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9151727/
https://www.ncbi.nlm.nih.gov/pubmed/35637227
http://dx.doi.org/10.1038/s41467-022-30515-0

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