Cationic copolymers that enhance wild-type-specific suppression in BNA-clamp PCR and preferentially increase the T(m) of fully matched complementary DNA and BNA strands

Mutation detection is of major interest in molecular diagnostics, especially in the field of oncology. However, detection can be challenging as mutant alleles often coexist with excess copies of wild-type alleles. Bridged nucleic acid (BNA)-clamp PCR circumvents this challenge by preferentially supp...

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Detalles Bibliográficos
Autores principales: Tachibana, Ami, Fujimura, Nahohiro, Takeuchi, Minoru, Watanabe, Koji, Teruuchi, Yoko, Uchiki, Tomoaki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2022
Materias:
Methods Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9154250/
https://www.ncbi.nlm.nih.gov/pubmed/35664806
http://dx.doi.org/10.1093/biomethods/bpac009

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9154250/
https://www.ncbi.nlm.nih.gov/pubmed/35664806
http://dx.doi.org/10.1093/biomethods/bpac009

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