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Cationic copolymers that enhance wild-type-specific suppression in BNA-clamp PCR and preferentially increase the T(m) of fully matched complementary DNA and BNA strands
Mutation detection is of major interest in molecular diagnostics, especially in the field of oncology. However, detection can be challenging as mutant alleles often coexist with excess copies of wild-type alleles. Bridged nucleic acid (BNA)-clamp PCR circumvents this challenge by preferentially supp...
| Autores principales: | , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Oxford University Press
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9154250/ https://www.ncbi.nlm.nih.gov/pubmed/35664806 http://dx.doi.org/10.1093/biomethods/bpac009 |