Modular (de)construction of complex bacterial phenotypes by CRISPR/nCas9-assisted, multiplex cytidine base-editing

CRISPR/Cas technologies constitute a powerful tool for genome engineering, yet their use in non-traditional bacteria depends on host factors or exogenous recombinases, which limits both efficiency and throughput. Here we mitigate these practical constraints by developing a widely-applicable genome e...

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Detalles Bibliográficos
Autores principales: Volke, Daniel C., Martino, Román A., Kozaeva, Ekaterina, Smania, Andrea M., Nikel, Pablo I.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9156665/
https://www.ncbi.nlm.nih.gov/pubmed/35641501
http://dx.doi.org/10.1038/s41467-022-30780-z

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9156665/
https://www.ncbi.nlm.nih.gov/pubmed/35641501
http://dx.doi.org/10.1038/s41467-022-30780-z

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