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Multiplex knockout of trichome-regulating MYB duplicates in hybrid poplar using a single gRNA

As the focus for CRISPR/Cas-edited plants moves from proof-of-concept to real-world applications, precise gene manipulation will increasingly require concurrent multiplex editing for polygenic traits. A common approach for editing across multiple sites is to design one guide RNA (gRNA) per target; h...

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Detalles Bibliográficos
Autores principales: Bewg, William P, Harding, Scott A, Engle, Nancy L, Vaidya, Brajesh N, Zhou, Ran, Reeves, Jacob, Horn, Thomas W, Joshee, Nirmal, Jenkins, Jerry W, Shu, Shengqiang, Barry, Kerrie W, Yoshinaga, Yuko, Grimwood, Jane, Schmitz, Robert J, Schmutz, Jeremy, Tschaplinski, Timothy J, Tsai, Chung-Jui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9157173/
https://www.ncbi.nlm.nih.gov/pubmed/35298644
http://dx.doi.org/10.1093/plphys/kiac128