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Multiplex knockout of trichome-regulating MYB duplicates in hybrid poplar using a single gRNA
As the focus for CRISPR/Cas-edited plants moves from proof-of-concept to real-world applications, precise gene manipulation will increasingly require concurrent multiplex editing for polygenic traits. A common approach for editing across multiple sites is to design one guide RNA (gRNA) per target; h...
Autores principales: | Bewg, William P, Harding, Scott A, Engle, Nancy L, Vaidya, Brajesh N, Zhou, Ran, Reeves, Jacob, Horn, Thomas W, Joshee, Nirmal, Jenkins, Jerry W, Shu, Shengqiang, Barry, Kerrie W, Yoshinaga, Yuko, Grimwood, Jane, Schmitz, Robert J, Schmutz, Jeremy, Tschaplinski, Timothy J, Tsai, Chung-Jui |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9157173/ https://www.ncbi.nlm.nih.gov/pubmed/35298644 http://dx.doi.org/10.1093/plphys/kiac128 |
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