Protocol for high-throughput cloning, expression, purification, and evaluation of bispecific antibodies

Bispecific antibodies are a powerful new class of therapeutics, but their development often requires enormous amounts of time and resources. Here, we describe a high-throughput protocol for cloning, expressing, purifying, and evaluating bispecific antibodies. This protocol enables the rapid screenin...

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Detalles Bibliográficos
Autores principales: Li, Danqing, Partin, Alexander C., Zhao, Liangjun, Chen, Irwin, Michaels, Mark L., Wang, Zhulun, Garces, Fernando, Gong, Danyang, Riley, Timothy P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9157557/
https://www.ncbi.nlm.nih.gov/pubmed/35664258
http://dx.doi.org/10.1016/j.xpro.2022.101428
Descripción
Sumario:Bispecific antibodies are a powerful new class of therapeutics, but their development often requires enormous amounts of time and resources. Here, we describe a high-throughput protocol for cloning, expressing, purifying, and evaluating bispecific antibodies. This protocol enables the rapid screening of large panels of bispecific molecules to identify top candidates for further development. For complete details on the use and execution of this protocol, please refer to Estes et al. (2021).

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