Isolation, cultivation, and application of primary respiratory epithelial cells obtained by nasal brushing, polyp samples, or lung explants

Here, we present a standardized protocol for isolation, maintenance, and polarization of the respiratory epithelial primary cells from patient samples acquired from nasal brushing, polyp specimens, or lung explants. This protocol generates a clearly defined polarized layer of epithelial cells on fil...

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Detalles Bibliográficos
Autores principales: Golec, Anita, Pranke, Iwona, Scudieri, Paolo, Hayes, Kate, Dreano, Elise, Dunlevy, Fiona, Hatton, Aurelie, Downey, Damian G., Galietta, Luis, Sermet, Isabelle
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9157560/
https://www.ncbi.nlm.nih.gov/pubmed/35664255
http://dx.doi.org/10.1016/j.xpro.2022.101419

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9157560/
https://www.ncbi.nlm.nih.gov/pubmed/35664255
http://dx.doi.org/10.1016/j.xpro.2022.101419

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