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Engineering high levels of saffron apocarotenoids in tomato

Crocins and picrocrocin are high-value hydrophilic pigments produced in saffron and used commercially in the food and pharmaceutical industries. These apocarotenoids are derived from the oxidative cleavage of zeaxanthin by specific carotenoid cleavage dioxygenases. The pathway for crocins and picroc...

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Autores principales: Ahrazem, Oussama, Diretto, Gianfranco, Rambla, José Luis, Rubio-Moraga, Ángela, Lobato-Gómez, María, Frusciante, Sarah, Argandoña, Javier, Presa, Silvia, Granell, Antonio, Gómez-Gómez, Lourdes
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9157650/
https://www.ncbi.nlm.nih.gov/pubmed/35669709
http://dx.doi.org/10.1093/hr/uhac074
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author Ahrazem, Oussama
Diretto, Gianfranco
Rambla, José Luis
Rubio-Moraga, Ángela
Lobato-Gómez, María
Frusciante, Sarah
Argandoña, Javier
Presa, Silvia
Granell, Antonio
Gómez-Gómez, Lourdes
author_facet Ahrazem, Oussama
Diretto, Gianfranco
Rambla, José Luis
Rubio-Moraga, Ángela
Lobato-Gómez, María
Frusciante, Sarah
Argandoña, Javier
Presa, Silvia
Granell, Antonio
Gómez-Gómez, Lourdes
author_sort Ahrazem, Oussama
collection PubMed
description Crocins and picrocrocin are high-value hydrophilic pigments produced in saffron and used commercially in the food and pharmaceutical industries. These apocarotenoids are derived from the oxidative cleavage of zeaxanthin by specific carotenoid cleavage dioxygenases. The pathway for crocins and picrocrocin biosynthesis was introduced into tomato using fruit specific and constitutive promoters and resulted in 14.48 mg/g of crocins and 2.92 mg/g of picrocrocin in the tomato DW, without compromising plant growth. The strategy involved expression of CsCCD2L to produce crocetin dialdehyde and 2,6,6-trimethyl-4-hydroxy-1-carboxaldehyde-1-cyclohexene, and of glycosyltransferases UGT709G1 and CsUGT2 for picrocrocin and crocins production, respectively. Metabolic analyses of the engineered fruits revealed picrocrocin and crocetin-(β-D-gentiobiosyl)-(β-D-glucosyl)-ester, as the predominant crocin molecule, as well as safranal, at the expense of the usual tomato carotenoids. The results showed the highest crocins content ever obtained by metabolic engineering in heterologous systems. In addition, the engineered tomatoes showed higher antioxidant capacity and were able to protect against neurological disorders in a Caenorhabditis elegans model of Alzheimer’s disease. Therefore, these new developed tomatoes could be exploited as a new platform to produce economically competitive saffron apocarotenoids with health-promoting properties.
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spelling pubmed-91576502022-06-05 Engineering high levels of saffron apocarotenoids in tomato Ahrazem, Oussama Diretto, Gianfranco Rambla, José Luis Rubio-Moraga, Ángela Lobato-Gómez, María Frusciante, Sarah Argandoña, Javier Presa, Silvia Granell, Antonio Gómez-Gómez, Lourdes Hortic Res Article Crocins and picrocrocin are high-value hydrophilic pigments produced in saffron and used commercially in the food and pharmaceutical industries. These apocarotenoids are derived from the oxidative cleavage of zeaxanthin by specific carotenoid cleavage dioxygenases. The pathway for crocins and picrocrocin biosynthesis was introduced into tomato using fruit specific and constitutive promoters and resulted in 14.48 mg/g of crocins and 2.92 mg/g of picrocrocin in the tomato DW, without compromising plant growth. The strategy involved expression of CsCCD2L to produce crocetin dialdehyde and 2,6,6-trimethyl-4-hydroxy-1-carboxaldehyde-1-cyclohexene, and of glycosyltransferases UGT709G1 and CsUGT2 for picrocrocin and crocins production, respectively. Metabolic analyses of the engineered fruits revealed picrocrocin and crocetin-(β-D-gentiobiosyl)-(β-D-glucosyl)-ester, as the predominant crocin molecule, as well as safranal, at the expense of the usual tomato carotenoids. The results showed the highest crocins content ever obtained by metabolic engineering in heterologous systems. In addition, the engineered tomatoes showed higher antioxidant capacity and were able to protect against neurological disorders in a Caenorhabditis elegans model of Alzheimer’s disease. Therefore, these new developed tomatoes could be exploited as a new platform to produce economically competitive saffron apocarotenoids with health-promoting properties. Oxford University Press 2022-03-23 /pmc/articles/PMC9157650/ /pubmed/35669709 http://dx.doi.org/10.1093/hr/uhac074 Text en © The Author(s) 2022. Published by Oxford University Press on behalf of Nanjing Agricultural University https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Article
Ahrazem, Oussama
Diretto, Gianfranco
Rambla, José Luis
Rubio-Moraga, Ángela
Lobato-Gómez, María
Frusciante, Sarah
Argandoña, Javier
Presa, Silvia
Granell, Antonio
Gómez-Gómez, Lourdes
Engineering high levels of saffron apocarotenoids in tomato
title Engineering high levels of saffron apocarotenoids in tomato
title_full Engineering high levels of saffron apocarotenoids in tomato
title_fullStr Engineering high levels of saffron apocarotenoids in tomato
title_full_unstemmed Engineering high levels of saffron apocarotenoids in tomato
title_short Engineering high levels of saffron apocarotenoids in tomato
title_sort engineering high levels of saffron apocarotenoids in tomato
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9157650/
https://www.ncbi.nlm.nih.gov/pubmed/35669709
http://dx.doi.org/10.1093/hr/uhac074
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