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An Integrated Metabolome and Transcriptome Analysis Reveal the Regulation Mechanisms of Flavonoid Biosynthesis in a Purple Tea Plant Cultivar

Purple tea plant cultivars, enrich with flavonoids and anthocyanins, are valuable materials for manufacturing tea with unique color and flavor. Researchers found that ‘Zijuan’ leaves changed from purple to green mainly caused by the decreased flavonoids and anthocyanins concentrations. The mechanism...

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Autores principales: Song, SaSa, Tao, Yu, Gao, LongHan, Liang, HuiLing, Tang, DeSong, Lin, Jie, Wang, YuChun, Gmitter, Frederick G., Li, ChunFang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9161209/
https://www.ncbi.nlm.nih.gov/pubmed/35665157
http://dx.doi.org/10.3389/fpls.2022.880227
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author Song, SaSa
Tao, Yu
Gao, LongHan
Liang, HuiLing
Tang, DeSong
Lin, Jie
Wang, YuChun
Gmitter, Frederick G.
Li, ChunFang
author_facet Song, SaSa
Tao, Yu
Gao, LongHan
Liang, HuiLing
Tang, DeSong
Lin, Jie
Wang, YuChun
Gmitter, Frederick G.
Li, ChunFang
author_sort Song, SaSa
collection PubMed
description Purple tea plant cultivars, enrich with flavonoids and anthocyanins, are valuable materials for manufacturing tea with unique color and flavor. Researchers found that ‘Zijuan’ leaves changed from purple to green mainly caused by the decreased flavonoids and anthocyanins concentrations. The mechanism of flavonoids and anthocyanin biosynthesis has been studied in many purple tea plant cultivars and the key genes which regulated the biosynthesis of flavonoid and anthocyanins in different purple tea plant cultivars were quite different. Also, the molecular regulation mechanism underlying the flavonoids and anthocyanins biosynthesis during leaves development and color changes is less-thoroughly understood. In this study, an integrative analysis of transcriptome and metabolome was performed on the purple leaves and green leaves of ‘Zijuan’ tea plant to reveal the regulatory networks correlated to flavonoid biosynthesis and to identify key regulatory genes. Our results indicated that the ‘Zijuan’ new shoots leaves were purple might be due to the copigmentation of quercetin and kaempferol derivatives. In ‘Zijuan’ tea plant cultivar, flavonoids metabolites concentrations in purple leaves and green leaves were significantly influenced by the genes involved in flavonoid biosynthesis, transcriptional regulation, transport, and hormone response. Transcription factors including NAC008, MYB23, and bHLH96 and transporters such as ABC transporter I might be responsible for the flavonoid and anthocyanins accumulation in purple leaves. This study provides a new insight into the metabolism and molecular mechanisms underlying flavonoid and anthocyanin biosynthesis in tea plant.
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spelling pubmed-91612092022-06-03 An Integrated Metabolome and Transcriptome Analysis Reveal the Regulation Mechanisms of Flavonoid Biosynthesis in a Purple Tea Plant Cultivar Song, SaSa Tao, Yu Gao, LongHan Liang, HuiLing Tang, DeSong Lin, Jie Wang, YuChun Gmitter, Frederick G. Li, ChunFang Front Plant Sci Plant Science Purple tea plant cultivars, enrich with flavonoids and anthocyanins, are valuable materials for manufacturing tea with unique color and flavor. Researchers found that ‘Zijuan’ leaves changed from purple to green mainly caused by the decreased flavonoids and anthocyanins concentrations. The mechanism of flavonoids and anthocyanin biosynthesis has been studied in many purple tea plant cultivars and the key genes which regulated the biosynthesis of flavonoid and anthocyanins in different purple tea plant cultivars were quite different. Also, the molecular regulation mechanism underlying the flavonoids and anthocyanins biosynthesis during leaves development and color changes is less-thoroughly understood. In this study, an integrative analysis of transcriptome and metabolome was performed on the purple leaves and green leaves of ‘Zijuan’ tea plant to reveal the regulatory networks correlated to flavonoid biosynthesis and to identify key regulatory genes. Our results indicated that the ‘Zijuan’ new shoots leaves were purple might be due to the copigmentation of quercetin and kaempferol derivatives. In ‘Zijuan’ tea plant cultivar, flavonoids metabolites concentrations in purple leaves and green leaves were significantly influenced by the genes involved in flavonoid biosynthesis, transcriptional regulation, transport, and hormone response. Transcription factors including NAC008, MYB23, and bHLH96 and transporters such as ABC transporter I might be responsible for the flavonoid and anthocyanins accumulation in purple leaves. This study provides a new insight into the metabolism and molecular mechanisms underlying flavonoid and anthocyanin biosynthesis in tea plant. Frontiers Media S.A. 2022-05-19 /pmc/articles/PMC9161209/ /pubmed/35665157 http://dx.doi.org/10.3389/fpls.2022.880227 Text en Copyright © 2022 Song, Tao, Gao, Liang, Tang, Lin, Wang, Gmitter and Li. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Song, SaSa
Tao, Yu
Gao, LongHan
Liang, HuiLing
Tang, DeSong
Lin, Jie
Wang, YuChun
Gmitter, Frederick G.
Li, ChunFang
An Integrated Metabolome and Transcriptome Analysis Reveal the Regulation Mechanisms of Flavonoid Biosynthesis in a Purple Tea Plant Cultivar
title An Integrated Metabolome and Transcriptome Analysis Reveal the Regulation Mechanisms of Flavonoid Biosynthesis in a Purple Tea Plant Cultivar
title_full An Integrated Metabolome and Transcriptome Analysis Reveal the Regulation Mechanisms of Flavonoid Biosynthesis in a Purple Tea Plant Cultivar
title_fullStr An Integrated Metabolome and Transcriptome Analysis Reveal the Regulation Mechanisms of Flavonoid Biosynthesis in a Purple Tea Plant Cultivar
title_full_unstemmed An Integrated Metabolome and Transcriptome Analysis Reveal the Regulation Mechanisms of Flavonoid Biosynthesis in a Purple Tea Plant Cultivar
title_short An Integrated Metabolome and Transcriptome Analysis Reveal the Regulation Mechanisms of Flavonoid Biosynthesis in a Purple Tea Plant Cultivar
title_sort integrated metabolome and transcriptome analysis reveal the regulation mechanisms of flavonoid biosynthesis in a purple tea plant cultivar
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9161209/
https://www.ncbi.nlm.nih.gov/pubmed/35665157
http://dx.doi.org/10.3389/fpls.2022.880227
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