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Natural Compounds Tapinarof and Galactomyces Ferment Filtrate Downregulate IL-33 Expression via the AHR/IL-37 Axis in Human Keratinocytes

Interleukin (IL)-37 suppresses systemic and local inflammation. It is expressed in the epidermis, the external layer of the skin, and is decreased in inflammatory skin diseases including atopic dermatitis (AD) and psoriasis. Therefore, an agent applied topically on the skin that can increase IL-37 c...

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Autores principales: Tsuji, Gaku, Hashimoto-Hachiya, Akiko, Matsuda-Taniguchi, Tomoyo, Takai-Yumine, Ayako, Takemura, Masaki, Yan, Xianghong, Furue, Masutaka, Nakahara, Takeshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9161696/
https://www.ncbi.nlm.nih.gov/pubmed/35663970
http://dx.doi.org/10.3389/fimmu.2022.745997
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author Tsuji, Gaku
Hashimoto-Hachiya, Akiko
Matsuda-Taniguchi, Tomoyo
Takai-Yumine, Ayako
Takemura, Masaki
Yan, Xianghong
Furue, Masutaka
Nakahara, Takeshi
author_facet Tsuji, Gaku
Hashimoto-Hachiya, Akiko
Matsuda-Taniguchi, Tomoyo
Takai-Yumine, Ayako
Takemura, Masaki
Yan, Xianghong
Furue, Masutaka
Nakahara, Takeshi
author_sort Tsuji, Gaku
collection PubMed
description Interleukin (IL)-37 suppresses systemic and local inflammation. It is expressed in the epidermis, the external layer of the skin, and is decreased in inflammatory skin diseases including atopic dermatitis (AD) and psoriasis. Therefore, an agent applied topically on the skin that can increase IL-37 could be promising for treating AD and psoriasis; however, the mechanism regulating IL-37 remains largely unknown. Given that IL-37 expression is induced in differentiated keratinocytes, a major component of the epidermis, and that activation of aryl hydrocarbon receptor (AHR), a ligand-activated transcription factor, promotes keratinocyte differentiation, we hypothesized that AHR might be involved in the IL-37 expression in human keratinocytes. We analyzed normal epidermal human keratinocytes (NHEKs) treated with tapinarof and Galactomyces ferment filtrate (GFF), which are potent AHR modulators. We found that tapinarof and GFF upregulated IL-37 in NHEKs, which was canceled by the knockdown of AHR using siRNA transfection, indicating that AHR mediates IL-37 expression in NHEKs. Furthermore, we found that the knockdown of IL-37 resulted in the upregulation of IL-33, an alarmin cytokine with crucial roles in the pathogenesis of AD and psoriasis. These findings suggest that IL-37 negatively regulates IL-33 expression in NHEKs. Finally, we examined whether tapinarof and GFF treatment modulates IL-33 expression in NHEKs. Such treatment inhibited IL-33 expression, which was partially reversed by the knockdown of either AHR or IL-37. Taken together, our findings provide the first evidence that tapinarof and GFF could have potential to prevent IL-33-overexpressing disorders such as AD and psoriasis via the AHR/IL-37 axis.
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spelling pubmed-91616962022-06-03 Natural Compounds Tapinarof and Galactomyces Ferment Filtrate Downregulate IL-33 Expression via the AHR/IL-37 Axis in Human Keratinocytes Tsuji, Gaku Hashimoto-Hachiya, Akiko Matsuda-Taniguchi, Tomoyo Takai-Yumine, Ayako Takemura, Masaki Yan, Xianghong Furue, Masutaka Nakahara, Takeshi Front Immunol Immunology Interleukin (IL)-37 suppresses systemic and local inflammation. It is expressed in the epidermis, the external layer of the skin, and is decreased in inflammatory skin diseases including atopic dermatitis (AD) and psoriasis. Therefore, an agent applied topically on the skin that can increase IL-37 could be promising for treating AD and psoriasis; however, the mechanism regulating IL-37 remains largely unknown. Given that IL-37 expression is induced in differentiated keratinocytes, a major component of the epidermis, and that activation of aryl hydrocarbon receptor (AHR), a ligand-activated transcription factor, promotes keratinocyte differentiation, we hypothesized that AHR might be involved in the IL-37 expression in human keratinocytes. We analyzed normal epidermal human keratinocytes (NHEKs) treated with tapinarof and Galactomyces ferment filtrate (GFF), which are potent AHR modulators. We found that tapinarof and GFF upregulated IL-37 in NHEKs, which was canceled by the knockdown of AHR using siRNA transfection, indicating that AHR mediates IL-37 expression in NHEKs. Furthermore, we found that the knockdown of IL-37 resulted in the upregulation of IL-33, an alarmin cytokine with crucial roles in the pathogenesis of AD and psoriasis. These findings suggest that IL-37 negatively regulates IL-33 expression in NHEKs. Finally, we examined whether tapinarof and GFF treatment modulates IL-33 expression in NHEKs. Such treatment inhibited IL-33 expression, which was partially reversed by the knockdown of either AHR or IL-37. Taken together, our findings provide the first evidence that tapinarof and GFF could have potential to prevent IL-33-overexpressing disorders such as AD and psoriasis via the AHR/IL-37 axis. Frontiers Media S.A. 2022-05-19 /pmc/articles/PMC9161696/ /pubmed/35663970 http://dx.doi.org/10.3389/fimmu.2022.745997 Text en Copyright © 2022 Tsuji, Hashimoto-Hachiya, Matsuda-Taniguchi, Takai-Yumine, Takemura, Yan, Furue and Nakahara https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Tsuji, Gaku
Hashimoto-Hachiya, Akiko
Matsuda-Taniguchi, Tomoyo
Takai-Yumine, Ayako
Takemura, Masaki
Yan, Xianghong
Furue, Masutaka
Nakahara, Takeshi
Natural Compounds Tapinarof and Galactomyces Ferment Filtrate Downregulate IL-33 Expression via the AHR/IL-37 Axis in Human Keratinocytes
title Natural Compounds Tapinarof and Galactomyces Ferment Filtrate Downregulate IL-33 Expression via the AHR/IL-37 Axis in Human Keratinocytes
title_full Natural Compounds Tapinarof and Galactomyces Ferment Filtrate Downregulate IL-33 Expression via the AHR/IL-37 Axis in Human Keratinocytes
title_fullStr Natural Compounds Tapinarof and Galactomyces Ferment Filtrate Downregulate IL-33 Expression via the AHR/IL-37 Axis in Human Keratinocytes
title_full_unstemmed Natural Compounds Tapinarof and Galactomyces Ferment Filtrate Downregulate IL-33 Expression via the AHR/IL-37 Axis in Human Keratinocytes
title_short Natural Compounds Tapinarof and Galactomyces Ferment Filtrate Downregulate IL-33 Expression via the AHR/IL-37 Axis in Human Keratinocytes
title_sort natural compounds tapinarof and galactomyces ferment filtrate downregulate il-33 expression via the ahr/il-37 axis in human keratinocytes
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9161696/
https://www.ncbi.nlm.nih.gov/pubmed/35663970
http://dx.doi.org/10.3389/fimmu.2022.745997
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