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Bone Mesenchymal Stem Cell-Derived Exosome-Enclosed miR-181a Induces CD4(+)CD25(+)FOXP3(+) Regulatory T Cells via SIRT1/Acetylation-Mediated FOXP3 Stabilization

Bone marrow mesenchymal stem cells (BMSCs) have been identified as a potential therapeutic approach to immune-related diseases. Here, we show that BMSC-derived exosomes promote FOXP3 expression and induce the conversion of CD4(+) T cells into CD4(+)CD25(+)FOXP3(+) Treg cells, which is significant fo...

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Autores principales: Wang, Renyong, Li, Ruixue, Li, Tiehan, Zhu, Lei, Qi, Zongze, Yang, Xiaokui, Wang, Huan, Cao, Baoquan, Zhu, Hong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9162841/
https://www.ncbi.nlm.nih.gov/pubmed/35664563
http://dx.doi.org/10.1155/2022/8890434
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author Wang, Renyong
Li, Ruixue
Li, Tiehan
Zhu, Lei
Qi, Zongze
Yang, Xiaokui
Wang, Huan
Cao, Baoquan
Zhu, Hong
author_facet Wang, Renyong
Li, Ruixue
Li, Tiehan
Zhu, Lei
Qi, Zongze
Yang, Xiaokui
Wang, Huan
Cao, Baoquan
Zhu, Hong
author_sort Wang, Renyong
collection PubMed
description Bone marrow mesenchymal stem cells (BMSCs) have been identified as a potential therapeutic approach to immune-related diseases. Here, we show that BMSC-derived exosomes promote FOXP3 expression and induce the conversion of CD4(+) T cells into CD4(+)CD25(+)FOXP3(+) Treg cells, which is significant for immunosuppressive activity. We found that miR-181a-5p is upregulated in BMSC-derived exosomes and can be transferred to CD4(+) T cells. In CD4(+) cells, miR-181a directly targets SIRT1 and suppresses its expression. Moreover, downregulated SIRT1 enhances FOXP3 via protein acetylation. In conclusion, our data demonstrated that BMSC-derived exosomal miR-181a is critical in the maintenance of immune tolerance. Furthermore, our results reveal that BMSC-derived exosomal miR-181a induces the production of CD4(+)CD25(+)FOXP3(+) Treg cells via SIRT1/acetylation/FOXP3.
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spelling pubmed-91628412022-06-03 Bone Mesenchymal Stem Cell-Derived Exosome-Enclosed miR-181a Induces CD4(+)CD25(+)FOXP3(+) Regulatory T Cells via SIRT1/Acetylation-Mediated FOXP3 Stabilization Wang, Renyong Li, Ruixue Li, Tiehan Zhu, Lei Qi, Zongze Yang, Xiaokui Wang, Huan Cao, Baoquan Zhu, Hong J Oncol Research Article Bone marrow mesenchymal stem cells (BMSCs) have been identified as a potential therapeutic approach to immune-related diseases. Here, we show that BMSC-derived exosomes promote FOXP3 expression and induce the conversion of CD4(+) T cells into CD4(+)CD25(+)FOXP3(+) Treg cells, which is significant for immunosuppressive activity. We found that miR-181a-5p is upregulated in BMSC-derived exosomes and can be transferred to CD4(+) T cells. In CD4(+) cells, miR-181a directly targets SIRT1 and suppresses its expression. Moreover, downregulated SIRT1 enhances FOXP3 via protein acetylation. In conclusion, our data demonstrated that BMSC-derived exosomal miR-181a is critical in the maintenance of immune tolerance. Furthermore, our results reveal that BMSC-derived exosomal miR-181a induces the production of CD4(+)CD25(+)FOXP3(+) Treg cells via SIRT1/acetylation/FOXP3. Hindawi 2022-05-26 /pmc/articles/PMC9162841/ /pubmed/35664563 http://dx.doi.org/10.1155/2022/8890434 Text en Copyright © 2022 Renyong Wang et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Wang, Renyong
Li, Ruixue
Li, Tiehan
Zhu, Lei
Qi, Zongze
Yang, Xiaokui
Wang, Huan
Cao, Baoquan
Zhu, Hong
Bone Mesenchymal Stem Cell-Derived Exosome-Enclosed miR-181a Induces CD4(+)CD25(+)FOXP3(+) Regulatory T Cells via SIRT1/Acetylation-Mediated FOXP3 Stabilization
title Bone Mesenchymal Stem Cell-Derived Exosome-Enclosed miR-181a Induces CD4(+)CD25(+)FOXP3(+) Regulatory T Cells via SIRT1/Acetylation-Mediated FOXP3 Stabilization
title_full Bone Mesenchymal Stem Cell-Derived Exosome-Enclosed miR-181a Induces CD4(+)CD25(+)FOXP3(+) Regulatory T Cells via SIRT1/Acetylation-Mediated FOXP3 Stabilization
title_fullStr Bone Mesenchymal Stem Cell-Derived Exosome-Enclosed miR-181a Induces CD4(+)CD25(+)FOXP3(+) Regulatory T Cells via SIRT1/Acetylation-Mediated FOXP3 Stabilization
title_full_unstemmed Bone Mesenchymal Stem Cell-Derived Exosome-Enclosed miR-181a Induces CD4(+)CD25(+)FOXP3(+) Regulatory T Cells via SIRT1/Acetylation-Mediated FOXP3 Stabilization
title_short Bone Mesenchymal Stem Cell-Derived Exosome-Enclosed miR-181a Induces CD4(+)CD25(+)FOXP3(+) Regulatory T Cells via SIRT1/Acetylation-Mediated FOXP3 Stabilization
title_sort bone mesenchymal stem cell-derived exosome-enclosed mir-181a induces cd4(+)cd25(+)foxp3(+) regulatory t cells via sirt1/acetylation-mediated foxp3 stabilization
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9162841/
https://www.ncbi.nlm.nih.gov/pubmed/35664563
http://dx.doi.org/10.1155/2022/8890434
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