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High-resolution immunofluorescence imaging of mouse cochlear hair bundles

High-resolution immunofluorescence imaging of cochlear hair bundles faces many challenges due to the hair bundle’s small dimensions, fragile nature, and complex organization. Here, we describe an optimized protocol for hair-bundle protein immunostaining and localization. We detail the steps and solu...

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Detalles Bibliográficos
Autores principales: Miller, Katharine K., Wang, Pei, Grillet, Nicolas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9163841/
https://www.ncbi.nlm.nih.gov/pubmed/35669049
http://dx.doi.org/10.1016/j.xpro.2022.101431