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Validation Study to Determine the Accuracy of Widespread Promoterless EGFP Reporter at Assessing CRISPR/Cas9-Mediated Homology Directed Repair

An accurate visual reporter system to assess homology-directed repair (HDR) is a key prerequisite for evaluating the efficiency of Cas9-mediated precise gene editing. Herein, we tested the utility of the widespread promoterless EGFP reporter to assess the efficiency of CRISPR/Cas9-mediated homologou...

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Detalles Bibliográficos
Autores principales: Xu, Wanqing, Zuo, Qingxia, Feng, Dongyan, He, Changsheng, Lin, Cailing, Huang, Dongchao, Wan, Yanbin, Chen, Feng, Mo, Guosheng, Sun, Qi, Du, Hongli, Huang, Lizhen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9164083/
https://www.ncbi.nlm.nih.gov/pubmed/35723374
http://dx.doi.org/10.3390/cimb44040116