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Efficient and iterative retron-mediated in vivo recombineering in Escherichia coli

Recombineering is an important tool in gene editing, enabling fast, precise and highly specific in vivo modification of microbial genomes. Oligonucleotide-mediated recombineering via the in vivo production of single-stranded DNA can overcome the limitations of traditional recombineering methods that...

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Detalles Bibliográficos
Autores principales: Ellington, Adam J, Reisch, Christopher R
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9165427/
https://www.ncbi.nlm.nih.gov/pubmed/35673614
http://dx.doi.org/10.1093/synbio/ysac007