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Efficient and iterative retron-mediated in vivo recombineering in Escherichia coli
Recombineering is an important tool in gene editing, enabling fast, precise and highly specific in vivo modification of microbial genomes. Oligonucleotide-mediated recombineering via the in vivo production of single-stranded DNA can overcome the limitations of traditional recombineering methods that...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Oxford University Press
2022
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9165427/ https://www.ncbi.nlm.nih.gov/pubmed/35673614 http://dx.doi.org/10.1093/synbio/ysac007 |