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Workflow for high-dimensional flow cytometry analysis of T cells from tumor metastases

We describe a multi-step high-dimensional (HD) flow cytometry workflow for the deep phenotypic characterization of T cells infiltrating metastatic tumor lesions in the liver, particularly derived from colorectal cancer (CRC-LM). First, we applied a novel flow cytometer setting approach based on sing...

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Detalles Bibliográficos
Autores principales: Faccani, Cristina, Rotta, Gianluca, Clemente, Francesca, Fedeli, Maya, Abbati, Danilo, Manfredi, Francesco, Potenza, Alessia, Anselmo, Achille, Pedica, Federica, Fiorentini, Guido, Villa, Chiara, Protti, Maria P, Doglioni, Claudio, Aldrighetti, Luca, Bonini, Chiara, Casorati, Giulia, Dellabona, Paolo, de Lalla, Claudia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Life Science Alliance LLC 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9166301/
https://www.ncbi.nlm.nih.gov/pubmed/35724271
http://dx.doi.org/10.26508/lsa.202101316