RNF219 regulates CCR4-NOT function in mRNA translation and deadenylation

Post-transcriptional regulatory mechanisms play a role in many biological contexts through the control of mRNA degradation, translation and localization. Here, we show that the RING finger protein RNF219 co-purifies with the CCR4-NOT complex, the major mRNA deadenylase in eukaryotes, which mediates...

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Autores principales: Guénolé, Aude, Velilla, Fabien, Chartier, Aymeric, Rich, April, Carvunis, Anne-Ruxandra, Sardet, Claude, Simonelig, Martine, Sobhian, Bijan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9166816/
https://www.ncbi.nlm.nih.gov/pubmed/35660762
http://dx.doi.org/10.1038/s41598-022-13309-8
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author Guénolé, Aude
Velilla, Fabien
Chartier, Aymeric
Rich, April
Carvunis, Anne-Ruxandra
Sardet, Claude
Simonelig, Martine
Sobhian, Bijan
author_facet Guénolé, Aude
Velilla, Fabien
Chartier, Aymeric
Rich, April
Carvunis, Anne-Ruxandra
Sardet, Claude
Simonelig, Martine
Sobhian, Bijan
author_sort Guénolé, Aude
collection PubMed
description Post-transcriptional regulatory mechanisms play a role in many biological contexts through the control of mRNA degradation, translation and localization. Here, we show that the RING finger protein RNF219 co-purifies with the CCR4-NOT complex, the major mRNA deadenylase in eukaryotes, which mediates translational repression in both a deadenylase activity-dependent and -independent manner. Strikingly, RNF219 both inhibits the deadenylase activity of CCR4-NOT and enhances its capacity to repress translation of a target mRNA. We propose that the interaction of RNF219 with the CCR4-NOT complex directs the translational repressive activity of CCR4-NOT to a deadenylation-independent mechanism.
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spelling pubmed-91668162022-06-05 RNF219 regulates CCR4-NOT function in mRNA translation and deadenylation Guénolé, Aude Velilla, Fabien Chartier, Aymeric Rich, April Carvunis, Anne-Ruxandra Sardet, Claude Simonelig, Martine Sobhian, Bijan Sci Rep Article Post-transcriptional regulatory mechanisms play a role in many biological contexts through the control of mRNA degradation, translation and localization. Here, we show that the RING finger protein RNF219 co-purifies with the CCR4-NOT complex, the major mRNA deadenylase in eukaryotes, which mediates translational repression in both a deadenylase activity-dependent and -independent manner. Strikingly, RNF219 both inhibits the deadenylase activity of CCR4-NOT and enhances its capacity to repress translation of a target mRNA. We propose that the interaction of RNF219 with the CCR4-NOT complex directs the translational repressive activity of CCR4-NOT to a deadenylation-independent mechanism. Nature Publishing Group UK 2022-06-03 /pmc/articles/PMC9166816/ /pubmed/35660762 http://dx.doi.org/10.1038/s41598-022-13309-8 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Guénolé, Aude
Velilla, Fabien
Chartier, Aymeric
Rich, April
Carvunis, Anne-Ruxandra
Sardet, Claude
Simonelig, Martine
Sobhian, Bijan
RNF219 regulates CCR4-NOT function in mRNA translation and deadenylation
title RNF219 regulates CCR4-NOT function in mRNA translation and deadenylation
title_full RNF219 regulates CCR4-NOT function in mRNA translation and deadenylation
title_fullStr RNF219 regulates CCR4-NOT function in mRNA translation and deadenylation
title_full_unstemmed RNF219 regulates CCR4-NOT function in mRNA translation and deadenylation
title_short RNF219 regulates CCR4-NOT function in mRNA translation and deadenylation
title_sort rnf219 regulates ccr4-not function in mrna translation and deadenylation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9166816/
https://www.ncbi.nlm.nih.gov/pubmed/35660762
http://dx.doi.org/10.1038/s41598-022-13309-8
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