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A cellular thermal shift assay for detecting amino acid sites involved in drug target engagement
Evaluating how drugs bind to specific targets at the cellular level is essential to their development and efficacy. Here, we present a protocol to detect the amino acid sites involved in drug-target engagement using a cellular thermal shift assay, which is based on ligand-induced protein thermal sta...
| Autores principales: | , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9168144/ https://www.ncbi.nlm.nih.gov/pubmed/35677609 http://dx.doi.org/10.1016/j.xpro.2022.101423 |