Luciferase assay system to monitor fibroblast growth factor signal disruption in human iPSCs

We describe a protocol for a live-cell luciferase assay system for continuously monitoring fibroblast growth factor (FGF) signal disruption in human-induced pluripotent stem cells (iPSCs). Signal disrupting effects of chemicals are used as an indicator to evaluate toxicity. The assay is reliably pre...

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Detalles Bibliográficos
Autores principales: Kanno, Seiya, Mizota, Kashu, Okubo, Yusuke, Kageyama, Tatsuto, Yan, Lei, Fukuda, Junji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9168156/
https://www.ncbi.nlm.nih.gov/pubmed/35677614
http://dx.doi.org/10.1016/j.xpro.2022.101439

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9168156/
https://www.ncbi.nlm.nih.gov/pubmed/35677614
http://dx.doi.org/10.1016/j.xpro.2022.101439

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