Luciferase assay system to monitor fibroblast growth factor signal disruption in human iPSCs

We describe a protocol for a live-cell luciferase assay system for continuously monitoring fibroblast growth factor (FGF) signal disruption in human-induced pluripotent stem cells (iPSCs). Signal disrupting effects of chemicals are used as an indicator to evaluate toxicity. The assay is reliably pre...

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Detalles Bibliográficos
Autores principales: Kanno, Seiya, Mizota, Kashu, Okubo, Yusuke, Kageyama, Tatsuto, Yan, Lei, Fukuda, Junji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9168156/
https://www.ncbi.nlm.nih.gov/pubmed/35677614
http://dx.doi.org/10.1016/j.xpro.2022.101439
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author Kanno, Seiya
Mizota, Kashu
Okubo, Yusuke
Kageyama, Tatsuto
Yan, Lei
Fukuda, Junji
author_facet Kanno, Seiya
Mizota, Kashu
Okubo, Yusuke
Kageyama, Tatsuto
Yan, Lei
Fukuda, Junji
author_sort Kanno, Seiya
collection PubMed
description We describe a protocol for a live-cell luciferase assay system for continuously monitoring fibroblast growth factor (FGF) signal disruption in human-induced pluripotent stem cells (iPSCs). Signal disrupting effects of chemicals are used as an indicator to evaluate toxicity. The assay is reliably predictive of the effects of limb malformation chemicals (AUC = 0.93). The current approach is limited to FGF signal disruption, and combinations with other types of signaling will be required to detect the effects of different toxicants. For complete details on the use and execution of this protocol, please refer to Kanno et al. (2022a).
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spelling pubmed-91681562022-06-07 Luciferase assay system to monitor fibroblast growth factor signal disruption in human iPSCs Kanno, Seiya Mizota, Kashu Okubo, Yusuke Kageyama, Tatsuto Yan, Lei Fukuda, Junji STAR Protoc Protocol We describe a protocol for a live-cell luciferase assay system for continuously monitoring fibroblast growth factor (FGF) signal disruption in human-induced pluripotent stem cells (iPSCs). Signal disrupting effects of chemicals are used as an indicator to evaluate toxicity. The assay is reliably predictive of the effects of limb malformation chemicals (AUC = 0.93). The current approach is limited to FGF signal disruption, and combinations with other types of signaling will be required to detect the effects of different toxicants. For complete details on the use and execution of this protocol, please refer to Kanno et al. (2022a). Elsevier 2022-06-04 /pmc/articles/PMC9168156/ /pubmed/35677614 http://dx.doi.org/10.1016/j.xpro.2022.101439 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Kanno, Seiya
Mizota, Kashu
Okubo, Yusuke
Kageyama, Tatsuto
Yan, Lei
Fukuda, Junji
Luciferase assay system to monitor fibroblast growth factor signal disruption in human iPSCs
title Luciferase assay system to monitor fibroblast growth factor signal disruption in human iPSCs
title_full Luciferase assay system to monitor fibroblast growth factor signal disruption in human iPSCs
title_fullStr Luciferase assay system to monitor fibroblast growth factor signal disruption in human iPSCs
title_full_unstemmed Luciferase assay system to monitor fibroblast growth factor signal disruption in human iPSCs
title_short Luciferase assay system to monitor fibroblast growth factor signal disruption in human iPSCs
title_sort luciferase assay system to monitor fibroblast growth factor signal disruption in human ipscs
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9168156/
https://www.ncbi.nlm.nih.gov/pubmed/35677614
http://dx.doi.org/10.1016/j.xpro.2022.101439
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