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Luciferase assay system to monitor fibroblast growth factor signal disruption in human iPSCs

We describe a protocol for a live-cell luciferase assay system for continuously monitoring fibroblast growth factor (FGF) signal disruption in human-induced pluripotent stem cells (iPSCs). Signal disrupting effects of chemicals are used as an indicator to evaluate toxicity. The assay is reliably pre...

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Detalles Bibliográficos
Autores principales:	Kanno, Seiya, Mizota, Kashu, Okubo, Yusuke, Kageyama, Tatsuto, Yan, Lei, Fukuda, Junji
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2022
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9168156/ https://www.ncbi.nlm.nih.gov/pubmed/35677614 http://dx.doi.org/10.1016/j.xpro.2022.101439

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