Generation of a dual edited human induced pluripotent stem cell Myl7-GFP reporter line with inducible CRISPRi/dCas9

Temporal regulation of CRISPRi activity is critical for genetic screens. Here, we present an inducible CRISPRi platform enabling selection of iPSC-derived cardiomyocytes and reversible gene knockdown. We targeted a doxycycline-inducible dCas9-KRAB-mCherry cassette into the AAVS1 locus in an MYL7-mGF...

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Detalles Bibliográficos
Autores principales: Metzl-Raz, Eyal, Bharucha, Nike, Ataam, Jennifer Arthur, Gavidia, Alexandra A., Greenleaf, William J., Karakikes, Ioannis
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9169598/
https://www.ncbi.nlm.nih.gov/pubmed/35325819
http://dx.doi.org/10.1016/j.scr.2022.102754

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9169598/
https://www.ncbi.nlm.nih.gov/pubmed/35325819
http://dx.doi.org/10.1016/j.scr.2022.102754

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