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Persistence of plasmids targeted by CRISPR interference in bacterial populations
CRISPR-Cas systems provide prokaryotes with an RNA-guided defense against foreign mobile genetic elements (MGEs) such as plasmids and viruses. A common mechanism by which MGEs avoid interference by CRISPR consists of acquisition of escape mutations in regions targeted by CRISPR. Here, using microbio...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
National Academy of Sciences
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9169639/ https://www.ncbi.nlm.nih.gov/pubmed/35394860 http://dx.doi.org/10.1073/pnas.2114905119 |
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author | Mamontov, Viktor Martynov, Alexander Morozova, Natalia Bukatin, Anton Staroverov, Dmitry B. Lukyanov, Konstantin A. Ispolatov, Yaroslav Semenova, Ekaterina Severinov, Konstantin |
author_facet | Mamontov, Viktor Martynov, Alexander Morozova, Natalia Bukatin, Anton Staroverov, Dmitry B. Lukyanov, Konstantin A. Ispolatov, Yaroslav Semenova, Ekaterina Severinov, Konstantin |
author_sort | Mamontov, Viktor |
collection | PubMed |
description | CRISPR-Cas systems provide prokaryotes with an RNA-guided defense against foreign mobile genetic elements (MGEs) such as plasmids and viruses. A common mechanism by which MGEs avoid interference by CRISPR consists of acquisition of escape mutations in regions targeted by CRISPR. Here, using microbiological, live microscopy and microfluidics analyses we demonstrate that plasmids can persist for multiple generations in some Escherichia coli cell lineages at conditions of continuous targeting by the type I-E CRISPR-Cas system. We used mathematical modeling to show how plasmid persistence in a subpopulation of cells mounting CRISPR interference is achieved due to the stochastic nature of CRISPR interference and plasmid replication events. We hypothesize that the observed complex dynamics provides bacterial populations with long-term benefits due to continuous maintenance of mobile genetic elements in some cells, which leads to diversification of phenotypes in the entire community and allows rapid changes in the population structure to meet the demands of a changing environment. |
format | Online Article Text |
id | pubmed-9169639 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | National Academy of Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-91696392022-10-08 Persistence of plasmids targeted by CRISPR interference in bacterial populations Mamontov, Viktor Martynov, Alexander Morozova, Natalia Bukatin, Anton Staroverov, Dmitry B. Lukyanov, Konstantin A. Ispolatov, Yaroslav Semenova, Ekaterina Severinov, Konstantin Proc Natl Acad Sci U S A Biological Sciences CRISPR-Cas systems provide prokaryotes with an RNA-guided defense against foreign mobile genetic elements (MGEs) such as plasmids and viruses. A common mechanism by which MGEs avoid interference by CRISPR consists of acquisition of escape mutations in regions targeted by CRISPR. Here, using microbiological, live microscopy and microfluidics analyses we demonstrate that plasmids can persist for multiple generations in some Escherichia coli cell lineages at conditions of continuous targeting by the type I-E CRISPR-Cas system. We used mathematical modeling to show how plasmid persistence in a subpopulation of cells mounting CRISPR interference is achieved due to the stochastic nature of CRISPR interference and plasmid replication events. We hypothesize that the observed complex dynamics provides bacterial populations with long-term benefits due to continuous maintenance of mobile genetic elements in some cells, which leads to diversification of phenotypes in the entire community and allows rapid changes in the population structure to meet the demands of a changing environment. National Academy of Sciences 2022-04-08 2022-04-12 /pmc/articles/PMC9169639/ /pubmed/35394860 http://dx.doi.org/10.1073/pnas.2114905119 Text en Copyright © 2022 the Author(s). Published by PNAS. https://creativecommons.org/licenses/by-nc-nd/4.0/This article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND) (https://creativecommons.org/licenses/by-nc-nd/4.0/) . |
spellingShingle | Biological Sciences Mamontov, Viktor Martynov, Alexander Morozova, Natalia Bukatin, Anton Staroverov, Dmitry B. Lukyanov, Konstantin A. Ispolatov, Yaroslav Semenova, Ekaterina Severinov, Konstantin Persistence of plasmids targeted by CRISPR interference in bacterial populations |
title | Persistence of plasmids targeted by CRISPR interference in bacterial populations |
title_full | Persistence of plasmids targeted by CRISPR interference in bacterial populations |
title_fullStr | Persistence of plasmids targeted by CRISPR interference in bacterial populations |
title_full_unstemmed | Persistence of plasmids targeted by CRISPR interference in bacterial populations |
title_short | Persistence of plasmids targeted by CRISPR interference in bacterial populations |
title_sort | persistence of plasmids targeted by crispr interference in bacterial populations |
topic | Biological Sciences |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9169639/ https://www.ncbi.nlm.nih.gov/pubmed/35394860 http://dx.doi.org/10.1073/pnas.2114905119 |
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