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Acat1/Soat1 knockout extends the mutant Npc1 mouse lifespan and ameliorates functional deficiencies in multiple organelles of mutant cells

Multiple membrane organelles require cholesterol for proper function within cells. The Niemann-Pick type C (NPC) proteins export cholesterol from endosomes to other membrane compartments, including the endoplasmic reticulum (ER), plasma membrane (PM), trans-Golgi network (TGN), and mitochondria, to...

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Autores principales: Rogers, Maximillian A., Chang, Catherine C. Y., Maue, Robert A., Melton, Elaina M., Peden, Andrew A., Garver, William S., Lee, Junghoon, Schroen, Peter, Huang, Mitchell, Chang, Ta-Yuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: National Academy of Sciences 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9170141/
https://www.ncbi.nlm.nih.gov/pubmed/35507892
http://dx.doi.org/10.1073/pnas.2201646119
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author Rogers, Maximillian A.
Chang, Catherine C. Y.
Maue, Robert A.
Melton, Elaina M.
Peden, Andrew A.
Garver, William S.
Lee, Junghoon
Schroen, Peter
Huang, Mitchell
Chang, Ta-Yuan
author_facet Rogers, Maximillian A.
Chang, Catherine C. Y.
Maue, Robert A.
Melton, Elaina M.
Peden, Andrew A.
Garver, William S.
Lee, Junghoon
Schroen, Peter
Huang, Mitchell
Chang, Ta-Yuan
author_sort Rogers, Maximillian A.
collection PubMed
description Multiple membrane organelles require cholesterol for proper function within cells. The Niemann-Pick type C (NPC) proteins export cholesterol from endosomes to other membrane compartments, including the endoplasmic reticulum (ER), plasma membrane (PM), trans-Golgi network (TGN), and mitochondria, to meet their cholesterol requirements. Defects in NPC cause malfunctions in multiple membrane organelles and lead to an incurable neurological disorder. Acyl-coenzyme A:cholesterol acyltransferase 1 (ACAT1), a resident enzyme in the ER, converts cholesterol to cholesteryl esters for storage. In mutant NPC cells, cholesterol storage still occurs in an NPC-independent manner. Here we report the interesting finding that in a mutant Npc1 mouse (Npc1(nmf)), Acat1 gene (Soat1) knockout delayed the onset of weight loss, motor impairment, and Purkinje neuron death. It also improved hepatosplenic pathology and prolonged lifespan by 34%. In mutant NPC1 fibroblasts, ACAT1 blockade (A1B) increased cholesterol content associated with TGN-rich membranes and mitochondria, while decreased cholesterol content associated with late endosomes. A1B also restored proper localization of syntaxin 6 and golgin 97 (key proteins in membrane trafficking at TGN) and improved the levels of cathepsin D (a key protease in lysosome and requires Golgi/endosome transport for maturation) and ABCA1 (a key protein controlling cholesterol release at PM). This work supports the hypothesis that diverting cholesterol from storage can benefit multiple diseases that involve cholesterol deficiencies in cell membranes.
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spelling pubmed-91701412022-11-04 Acat1/Soat1 knockout extends the mutant Npc1 mouse lifespan and ameliorates functional deficiencies in multiple organelles of mutant cells Rogers, Maximillian A. Chang, Catherine C. Y. Maue, Robert A. Melton, Elaina M. Peden, Andrew A. Garver, William S. Lee, Junghoon Schroen, Peter Huang, Mitchell Chang, Ta-Yuan Proc Natl Acad Sci U S A Biological Sciences Multiple membrane organelles require cholesterol for proper function within cells. The Niemann-Pick type C (NPC) proteins export cholesterol from endosomes to other membrane compartments, including the endoplasmic reticulum (ER), plasma membrane (PM), trans-Golgi network (TGN), and mitochondria, to meet their cholesterol requirements. Defects in NPC cause malfunctions in multiple membrane organelles and lead to an incurable neurological disorder. Acyl-coenzyme A:cholesterol acyltransferase 1 (ACAT1), a resident enzyme in the ER, converts cholesterol to cholesteryl esters for storage. In mutant NPC cells, cholesterol storage still occurs in an NPC-independent manner. Here we report the interesting finding that in a mutant Npc1 mouse (Npc1(nmf)), Acat1 gene (Soat1) knockout delayed the onset of weight loss, motor impairment, and Purkinje neuron death. It also improved hepatosplenic pathology and prolonged lifespan by 34%. In mutant NPC1 fibroblasts, ACAT1 blockade (A1B) increased cholesterol content associated with TGN-rich membranes and mitochondria, while decreased cholesterol content associated with late endosomes. A1B also restored proper localization of syntaxin 6 and golgin 97 (key proteins in membrane trafficking at TGN) and improved the levels of cathepsin D (a key protease in lysosome and requires Golgi/endosome transport for maturation) and ABCA1 (a key protein controlling cholesterol release at PM). This work supports the hypothesis that diverting cholesterol from storage can benefit multiple diseases that involve cholesterol deficiencies in cell membranes. National Academy of Sciences 2022-05-04 2022-05-03 /pmc/articles/PMC9170141/ /pubmed/35507892 http://dx.doi.org/10.1073/pnas.2201646119 Text en Copyright © 2022 the Author(s). Published by PNAS. https://creativecommons.org/licenses/by-nc-nd/4.0/This article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND) (https://creativecommons.org/licenses/by-nc-nd/4.0/) .
spellingShingle Biological Sciences
Rogers, Maximillian A.
Chang, Catherine C. Y.
Maue, Robert A.
Melton, Elaina M.
Peden, Andrew A.
Garver, William S.
Lee, Junghoon
Schroen, Peter
Huang, Mitchell
Chang, Ta-Yuan
Acat1/Soat1 knockout extends the mutant Npc1 mouse lifespan and ameliorates functional deficiencies in multiple organelles of mutant cells
title Acat1/Soat1 knockout extends the mutant Npc1 mouse lifespan and ameliorates functional deficiencies in multiple organelles of mutant cells
title_full Acat1/Soat1 knockout extends the mutant Npc1 mouse lifespan and ameliorates functional deficiencies in multiple organelles of mutant cells
title_fullStr Acat1/Soat1 knockout extends the mutant Npc1 mouse lifespan and ameliorates functional deficiencies in multiple organelles of mutant cells
title_full_unstemmed Acat1/Soat1 knockout extends the mutant Npc1 mouse lifespan and ameliorates functional deficiencies in multiple organelles of mutant cells
title_short Acat1/Soat1 knockout extends the mutant Npc1 mouse lifespan and ameliorates functional deficiencies in multiple organelles of mutant cells
title_sort acat1/soat1 knockout extends the mutant npc1 mouse lifespan and ameliorates functional deficiencies in multiple organelles of mutant cells
topic Biological Sciences
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9170141/
https://www.ncbi.nlm.nih.gov/pubmed/35507892
http://dx.doi.org/10.1073/pnas.2201646119
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