Cargando…

Investigation of the Potential Mechanisms Underlying Nuclear F-Actin Organization in Ovarian Cancer Cells by High-Throughput Screening in Combination With Deep Learning

Increasing evidence supports the notion that filamentous actin (F-actin) and globular actin exist in the nuclei of somatic cells, and are involved in chromatin remodeling, gene transcription regulation and DNA damage repair. However, the underlying mechanisms of how nuclear F-actin are polymerized i...

Descripción completa

Detalles Bibliográficos
Autores principales: Wu, Wei, Xing, Xiaoxia, Wang, Mingyang, Feng, Yinzhou, Wietek, Nina, Chong, Kay, El-Sahhar, Salma, Ahmed, Ahmed Ashour, Zang, Rongyu, Zheng, Yiyan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9178185/
https://www.ncbi.nlm.nih.gov/pubmed/35693931
http://dx.doi.org/10.3389/fcell.2022.869531
_version_ 1784723003124219904
author Wu, Wei
Xing, Xiaoxia
Wang, Mingyang
Feng, Yinzhou
Wietek, Nina
Chong, Kay
El-Sahhar, Salma
Ahmed, Ahmed Ashour
Zang, Rongyu
Zheng, Yiyan
author_facet Wu, Wei
Xing, Xiaoxia
Wang, Mingyang
Feng, Yinzhou
Wietek, Nina
Chong, Kay
El-Sahhar, Salma
Ahmed, Ahmed Ashour
Zang, Rongyu
Zheng, Yiyan
author_sort Wu, Wei
collection PubMed
description Increasing evidence supports the notion that filamentous actin (F-actin) and globular actin exist in the nuclei of somatic cells, and are involved in chromatin remodeling, gene transcription regulation and DNA damage repair. However, the underlying mechanisms of how nuclear F-actin are polymerized in cells remain incompletely understood. Here, we identify potential kinase targets that participate in nuclear F-actin polymerization in ovarian cancer cells using small-molecule inhibitor library screening in combination with a deep learning approach. The analysis of the targets of the inhibitors used in this study suggest that the PI3K-AKT pathway are involved in regulating nuclear F-actin organization in ovarian cancer cells. Our work lays the foundation for uncovering the important roles of nuclear F-actin in the context of ovarian cancer, and for understanding how nuclear F-actin structures are organized.
format Online
Article
Text
id pubmed-9178185
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-91781852022-06-10 Investigation of the Potential Mechanisms Underlying Nuclear F-Actin Organization in Ovarian Cancer Cells by High-Throughput Screening in Combination With Deep Learning Wu, Wei Xing, Xiaoxia Wang, Mingyang Feng, Yinzhou Wietek, Nina Chong, Kay El-Sahhar, Salma Ahmed, Ahmed Ashour Zang, Rongyu Zheng, Yiyan Front Cell Dev Biol Cell and Developmental Biology Increasing evidence supports the notion that filamentous actin (F-actin) and globular actin exist in the nuclei of somatic cells, and are involved in chromatin remodeling, gene transcription regulation and DNA damage repair. However, the underlying mechanisms of how nuclear F-actin are polymerized in cells remain incompletely understood. Here, we identify potential kinase targets that participate in nuclear F-actin polymerization in ovarian cancer cells using small-molecule inhibitor library screening in combination with a deep learning approach. The analysis of the targets of the inhibitors used in this study suggest that the PI3K-AKT pathway are involved in regulating nuclear F-actin organization in ovarian cancer cells. Our work lays the foundation for uncovering the important roles of nuclear F-actin in the context of ovarian cancer, and for understanding how nuclear F-actin structures are organized. Frontiers Media S.A. 2022-05-26 /pmc/articles/PMC9178185/ /pubmed/35693931 http://dx.doi.org/10.3389/fcell.2022.869531 Text en Copyright © 2022 Wu, Xing, Wang, Feng, Wietek, Chong, El-Sahhar, Ahmed, Zang and Zheng. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cell and Developmental Biology
Wu, Wei
Xing, Xiaoxia
Wang, Mingyang
Feng, Yinzhou
Wietek, Nina
Chong, Kay
El-Sahhar, Salma
Ahmed, Ahmed Ashour
Zang, Rongyu
Zheng, Yiyan
Investigation of the Potential Mechanisms Underlying Nuclear F-Actin Organization in Ovarian Cancer Cells by High-Throughput Screening in Combination With Deep Learning
title Investigation of the Potential Mechanisms Underlying Nuclear F-Actin Organization in Ovarian Cancer Cells by High-Throughput Screening in Combination With Deep Learning
title_full Investigation of the Potential Mechanisms Underlying Nuclear F-Actin Organization in Ovarian Cancer Cells by High-Throughput Screening in Combination With Deep Learning
title_fullStr Investigation of the Potential Mechanisms Underlying Nuclear F-Actin Organization in Ovarian Cancer Cells by High-Throughput Screening in Combination With Deep Learning
title_full_unstemmed Investigation of the Potential Mechanisms Underlying Nuclear F-Actin Organization in Ovarian Cancer Cells by High-Throughput Screening in Combination With Deep Learning
title_short Investigation of the Potential Mechanisms Underlying Nuclear F-Actin Organization in Ovarian Cancer Cells by High-Throughput Screening in Combination With Deep Learning
title_sort investigation of the potential mechanisms underlying nuclear f-actin organization in ovarian cancer cells by high-throughput screening in combination with deep learning
topic Cell and Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9178185/
https://www.ncbi.nlm.nih.gov/pubmed/35693931
http://dx.doi.org/10.3389/fcell.2022.869531
work_keys_str_mv AT wuwei investigationofthepotentialmechanismsunderlyingnuclearfactinorganizationinovariancancercellsbyhighthroughputscreeningincombinationwithdeeplearning
AT xingxiaoxia investigationofthepotentialmechanismsunderlyingnuclearfactinorganizationinovariancancercellsbyhighthroughputscreeningincombinationwithdeeplearning
AT wangmingyang investigationofthepotentialmechanismsunderlyingnuclearfactinorganizationinovariancancercellsbyhighthroughputscreeningincombinationwithdeeplearning
AT fengyinzhou investigationofthepotentialmechanismsunderlyingnuclearfactinorganizationinovariancancercellsbyhighthroughputscreeningincombinationwithdeeplearning
AT wieteknina investigationofthepotentialmechanismsunderlyingnuclearfactinorganizationinovariancancercellsbyhighthroughputscreeningincombinationwithdeeplearning
AT chongkay investigationofthepotentialmechanismsunderlyingnuclearfactinorganizationinovariancancercellsbyhighthroughputscreeningincombinationwithdeeplearning
AT elsahharsalma investigationofthepotentialmechanismsunderlyingnuclearfactinorganizationinovariancancercellsbyhighthroughputscreeningincombinationwithdeeplearning
AT ahmedahmedashour investigationofthepotentialmechanismsunderlyingnuclearfactinorganizationinovariancancercellsbyhighthroughputscreeningincombinationwithdeeplearning
AT zangrongyu investigationofthepotentialmechanismsunderlyingnuclearfactinorganizationinovariancancercellsbyhighthroughputscreeningincombinationwithdeeplearning
AT zhengyiyan investigationofthepotentialmechanismsunderlyingnuclearfactinorganizationinovariancancercellsbyhighthroughputscreeningincombinationwithdeeplearning