Cargando…
Deletion of the Autism-Associated Protein SHANK3 Abolishes Structural Synaptic Plasticity after Brain Trauma
Autism spectrum disorders (ASDs) are characterized by repetitive behaviors and impairments of sociability and communication. About 1% of ASD cases are caused by mutations of SHANK3, a major scaffolding protein of the postsynaptic density. We studied the role of SHANK3 in plastic changes of excitator...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9181590/ https://www.ncbi.nlm.nih.gov/pubmed/35682760 http://dx.doi.org/10.3390/ijms23116081 |
_version_ | 1784723814007963648 |
---|---|
author | Urrutia-Ruiz, Carolina Rombach, Daniel Cursano, Silvia Gerlach-Arbeiter, Susanne Schoen, Michael Bockmann, Juergen Demestre, Maria Boeckers, Tobias M. |
author_facet | Urrutia-Ruiz, Carolina Rombach, Daniel Cursano, Silvia Gerlach-Arbeiter, Susanne Schoen, Michael Bockmann, Juergen Demestre, Maria Boeckers, Tobias M. |
author_sort | Urrutia-Ruiz, Carolina |
collection | PubMed |
description | Autism spectrum disorders (ASDs) are characterized by repetitive behaviors and impairments of sociability and communication. About 1% of ASD cases are caused by mutations of SHANK3, a major scaffolding protein of the postsynaptic density. We studied the role of SHANK3 in plastic changes of excitatory synapses within the central nervous system by employing mild traumatic brain injury (mTBI) in WT and Shank3 knockout mice. In WT mice, mTBI triggered ipsi- and contralateral loss of hippocampal dendritic spines and excitatory synapses with a partial recovery over time. In contrast, no significant synaptic alterations were detected in Shank3∆11−/− mice, which showed fewer dendritic spines and excitatory synapses at baseline. In line, mTBI induced the upregulation of synaptic plasticity-related proteins Arc and p-cofilin only in WT mice. Interestingly, microglia proliferation was observed in WT mice after mTBI but not in Shank3∆11−/− mice. Finally, we detected TBI-induced increased fear memory at the behavioral level, whereas in Shank3∆11−/− animals, the already-enhanced fear memory levels increased only slightly after mTBI. Our data show the lack of structural synaptic plasticity in Shank3 knockout mice that might explain at least in part the rigidity of behaviors, problems in adjusting to new situations and cognitive deficits seen in ASDs. |
format | Online Article Text |
id | pubmed-9181590 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-91815902022-06-10 Deletion of the Autism-Associated Protein SHANK3 Abolishes Structural Synaptic Plasticity after Brain Trauma Urrutia-Ruiz, Carolina Rombach, Daniel Cursano, Silvia Gerlach-Arbeiter, Susanne Schoen, Michael Bockmann, Juergen Demestre, Maria Boeckers, Tobias M. Int J Mol Sci Article Autism spectrum disorders (ASDs) are characterized by repetitive behaviors and impairments of sociability and communication. About 1% of ASD cases are caused by mutations of SHANK3, a major scaffolding protein of the postsynaptic density. We studied the role of SHANK3 in plastic changes of excitatory synapses within the central nervous system by employing mild traumatic brain injury (mTBI) in WT and Shank3 knockout mice. In WT mice, mTBI triggered ipsi- and contralateral loss of hippocampal dendritic spines and excitatory synapses with a partial recovery over time. In contrast, no significant synaptic alterations were detected in Shank3∆11−/− mice, which showed fewer dendritic spines and excitatory synapses at baseline. In line, mTBI induced the upregulation of synaptic plasticity-related proteins Arc and p-cofilin only in WT mice. Interestingly, microglia proliferation was observed in WT mice after mTBI but not in Shank3∆11−/− mice. Finally, we detected TBI-induced increased fear memory at the behavioral level, whereas in Shank3∆11−/− animals, the already-enhanced fear memory levels increased only slightly after mTBI. Our data show the lack of structural synaptic plasticity in Shank3 knockout mice that might explain at least in part the rigidity of behaviors, problems in adjusting to new situations and cognitive deficits seen in ASDs. MDPI 2022-05-29 /pmc/articles/PMC9181590/ /pubmed/35682760 http://dx.doi.org/10.3390/ijms23116081 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Urrutia-Ruiz, Carolina Rombach, Daniel Cursano, Silvia Gerlach-Arbeiter, Susanne Schoen, Michael Bockmann, Juergen Demestre, Maria Boeckers, Tobias M. Deletion of the Autism-Associated Protein SHANK3 Abolishes Structural Synaptic Plasticity after Brain Trauma |
title | Deletion of the Autism-Associated Protein SHANK3 Abolishes Structural Synaptic Plasticity after Brain Trauma |
title_full | Deletion of the Autism-Associated Protein SHANK3 Abolishes Structural Synaptic Plasticity after Brain Trauma |
title_fullStr | Deletion of the Autism-Associated Protein SHANK3 Abolishes Structural Synaptic Plasticity after Brain Trauma |
title_full_unstemmed | Deletion of the Autism-Associated Protein SHANK3 Abolishes Structural Synaptic Plasticity after Brain Trauma |
title_short | Deletion of the Autism-Associated Protein SHANK3 Abolishes Structural Synaptic Plasticity after Brain Trauma |
title_sort | deletion of the autism-associated protein shank3 abolishes structural synaptic plasticity after brain trauma |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9181590/ https://www.ncbi.nlm.nih.gov/pubmed/35682760 http://dx.doi.org/10.3390/ijms23116081 |
work_keys_str_mv | AT urrutiaruizcarolina deletionoftheautismassociatedproteinshank3abolishesstructuralsynapticplasticityafterbraintrauma AT rombachdaniel deletionoftheautismassociatedproteinshank3abolishesstructuralsynapticplasticityafterbraintrauma AT cursanosilvia deletionoftheautismassociatedproteinshank3abolishesstructuralsynapticplasticityafterbraintrauma AT gerlacharbeitersusanne deletionoftheautismassociatedproteinshank3abolishesstructuralsynapticplasticityafterbraintrauma AT schoenmichael deletionoftheautismassociatedproteinshank3abolishesstructuralsynapticplasticityafterbraintrauma AT bockmannjuergen deletionoftheautismassociatedproteinshank3abolishesstructuralsynapticplasticityafterbraintrauma AT demestremaria deletionoftheautismassociatedproteinshank3abolishesstructuralsynapticplasticityafterbraintrauma AT boeckerstobiasm deletionoftheautismassociatedproteinshank3abolishesstructuralsynapticplasticityafterbraintrauma |