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Defining pervasive transcription units using chromatin RNA-sequencing data

Pervasive transcripts (PTs) are difficult to detect by steady-state RNA-seq, because they are degraded immediately by the nuclear exosome complex. Here, we describe a protocol illustrating a bioinformatic pipeline for genome-wide PTs de novo annotation via chromatin-associated RNA-seq data upon DIS3...

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Detalles Bibliográficos
Autores principales: Guo, Ziwei, Liu, Xinhong, Chen, Mo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9184797/
https://www.ncbi.nlm.nih.gov/pubmed/35693207
http://dx.doi.org/10.1016/j.xpro.2022.101442