Protein and antibody purification followed by immunoprecipitation of MYB and GATA zinc finger-type maize proteins with magnetic beads

Co-immunoprecipitation (Co-IP) is a widely used and powerful approach for studying protein-protein interactions in vivo. Here, we describe a protocol for antibody purification and immobilization followed by immunoprecipitation from plant tissue extracts using magnetic beads. The protocol has been us...

Descripción completa

Detalles Bibliográficos
Autores principales: Vélez-Bermúdez, Isabel Cristina, Salazar-Henao, Jorge Enrique, Riera, Marta, Caparros-Ruiz, David, Schmidt, Wolfgang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9184801/
https://www.ncbi.nlm.nih.gov/pubmed/35693212
http://dx.doi.org/10.1016/j.xpro.2022.101449

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9184801/
https://www.ncbi.nlm.nih.gov/pubmed/35693212
http://dx.doi.org/10.1016/j.xpro.2022.101449

Ejemplares similares