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Protein and antibody purification followed by immunoprecipitation of MYB and GATA zinc finger-type maize proteins with magnetic beads

Co-immunoprecipitation (Co-IP) is a widely used and powerful approach for studying protein-protein interactions in vivo. Here, we describe a protocol for antibody purification and immobilization followed by immunoprecipitation from plant tissue extracts using magnetic beads. The protocol has been us...

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Detalles Bibliográficos
Autores principales:	Vélez-Bermúdez, Isabel Cristina, Salazar-Henao, Jorge Enrique, Riera, Marta, Caparros-Ruiz, David, Schmidt, Wolfgang
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2022
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9184801/ https://www.ncbi.nlm.nih.gov/pubmed/35693212 http://dx.doi.org/10.1016/j.xpro.2022.101449

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